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ROTI®Sep 1077 human/tube

CELLPURE® sterile, ready-to-use, for density gradient centrifugation
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Pack Qty.
Pack.
Packaging
Lymphocyte separation medium, Polysucrose solution
Molar mass (M) ~400000 g/mol
Density (D) 1,077 g/cm³
Storage temp. +4 °C
WGK 1

Polysucrose based medium for separation of lymphocytes/PBMC from human whole blood. Density: 1,077 g/ml
15 ml prefilled in 50 ml tubes.
Long-term storage at approx. 4 °C recommended. Short-term storage at room temperature possible. Storage protected from light.

Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.
Product details
Type analysis
€395.00 /Pack Qty.

€307.90/Pack Qty.  Campaign price!

excl. VAT. | 25 unit(s) per Pack Qty.

Art. No. 0634.1

In stock
Free of shipping costs from 125 €
24 hour direct shipment
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Personal technical advice on this product
Dr Seipp
Dr Seipp
+49 721 5606 - 1038

Product details


Ready-to-use and sterile separation medium based on polysucrose 400, for efficient isolation of lymphocytes from human blood by density gradient centrifugation.
Density 1.077 g/ml.
Best alternative to Ficoll®.

  • Optimised for isolation of lymphocytes from non-coagulated human whole blood
  • Produces distinct, compact layers
  • Results in high recovery rates of viable cells
  • Maintains the representative ratio of B- to T lymphocytes
  • May be directly applied to all protocols using Ficoll®

ROTI®Sep 1077 human excels due to the superior separation power, forming very distinct, easy to pipet, lymphocyte layers during centrifugation. The ready to use solution is very easy in handling, resulting in high cell recovery rates, while cell viability and the representative ratio of B- to T lymphocytes are maintained. Osmolality as well as pH value are strictly kept in the physiological range.

Suitable for application with human non-coagulated peripheral blood. In all respective protocols originally using Ficoll® this reagent may be replaced by ROTI®Sep 1077 human without any adjustment.



ROTI®Sep 1077 human/tube CELLPURE® sterile, ready-to-use, for density gradient centrifugation

Prefilled in 50 ml centrifugation tubes, 15 ml each.

0634_Anwendung

With permeable membrane for easy pipetting of diluted blood.



Not a medical device / Not an IVD product
ROTI®Sep 1077 human/tube
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0634.1 25 unit(s) cardboard 25 x 50 ml €307.90
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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearance of solutionclear, colourless
Density1,077±0,001 g/ml
pH value7,15±0,35
Osmolality315±25 mOsm/kg
Endotoxinstested
Sterilitycomplies
For research use only. Not approved for human or veterinary use, for application to humans or animals, or for use in clinical or in vitro diagnostics.

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