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Coelenterazine, 2.5 mg

≥95 %, for biochemistry
Pack Qty.
Empirical formula C26H21N3O3
Molar mass (M) 423,48 g/mol
Melting point (mp) 178 °C
Storage temp. -20 °C
Transport temp. ambient temp.
CAS No. [55779-48-1]

Luciferase substrate

Substrate of the Renilla luciferase used for monitoring of the mitochondrial Calcium flux and of rep ...
Product details
Type analysis

€106.90/Pack Qty. 

excl. VAT. | 2.5 mg per Pack Qty.

Art. No. 4094.4

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Product details

Coelenterazine ≥95 %, for biochemistry

Substrate of the Renilla luciferase used for monitoring of the mitochondrial Calcium flux and of reportergenes. Coelenterazine is applied in bioluminescence resonance energy transfer (BRET) assays, ELISAs, HTS and in chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues.

Technical Information
Enzyme Luciferase 
Detection chemiluminescent 
Signal product soluble (ELISA, BRET) 
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4094.3 1 mg glass €56.90
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General information

Assay protocol for use of BCIP/NBT in immunoblot procedures:

Assay protocol for use of BCIP/NBT in immunoblot procedures:Stock solutions (All solutions are stable for at least 1 year at 4 °C):
0.5 g NBT in 10 ml 70 % dimethylformamide;
0.5 g BCIP (p-toluidine salt) in 10 ml 100 % dimethylformamide.
Incubation buffer for alkaline phosphatase:
100 mM NaCl, 5 mM MgCl2,100 mM Tris (pH 9.5).
Fresh substrate solution: 66 μl NBT stock solution + 10 ml incubation buffer, mix well, add 33 μl BCIP stock solution. Use within 1 hour.
Blot development: Approx. 10 ml substrate solution per 15 x 15 cm2 membrane surface. Develop at room temperature until bands become visible (approx. 30 mins).
Reaction stop: Rinse with PBS/20 mM EDTA.

Detection of peroxidase activity (Immunoassay):

Detection of peroxidase activity (Immunoassay):Dissolve 1 mg TMB in 0.1 ml dimethylsulfoxide (Art. No. 4720); add 9.9 ml of a 0.1 M sodium acetate solution (pH 6.0) (Powder: Art. No. 6779), filter and add H2O2 (Art. No. 8070) (final concentration 0.01 %).

Always prepare freshly!

Incubation 10-30 mins at room temperature (approx. 50 μl per microtitre well; finally add 50 μl 1 M H2SO4, Art. No. X873, per well).
Photometric quantitation at 450 nm.

Reference: Bos, E.S. et al., (1981) J. Immunoassay 2, (3/4), 187.

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Type analysis

Assay (TLC)≥95 %  

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