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ROTITEST®Vital, 1 ml, 1 x 1 ml

ROTITEST® Bio Analysis, sterile , ready-to-use
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
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Cell proliferation test solution
Storage temp. +4 °C
WGK 2

Colorimetric test solution for evaluation of cell proliferation and -viability. Non toxic.
Stabile at ambient temperature. Very stabile when being cooled.
Store protected from light. Do not freeze.

1 ml is sufficient for enumeration of 100 cell samples.

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.
Product details
Type analysis

€60.90/Pack Qty. 

excl. VAT. | 1 ml per Pack Qty.

Art. No. 0069.3

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Personal technical advice on this product
Ms Hübner
Ms Hübner
+49 721 5606 - 1034

Product details


Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.

Optimum replacement for MTT:
  • Cells keep on being vital
  • Most simple handling
  • One-component-system without radioactivity
  • For adherent and suspension cells
  • Photometric result in 1 to 4 hours
  • Strong correlation between absorbance and cell number
  • Suitable for every culture media


ROTITEST®Vital ROTITEST® Bio Analysis, sterile , ready-to-use
Mechanism

Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [3H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al., Anal. Commun. 1999 (36) 47-50).
In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST®vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.

0069_RotitestVital-Ablaufschema
0069_RotitestVital-Substanztest-Ablaufschema

Directions for use

The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm).
Also suitable for culture media containing phenol red.

Each package contains simple-to-follow instructions-for-use.

0069_Anwendung
Fig.: Example of use

0069_Diagramm_A
Fig. A: Correlation of cell counting results achieved with ROTITEST® Vital (10 μl/well) and 3H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM).
0069_Diagramm_CD
Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST® Vital (10 μl/well).

Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST® Vital (10 μl/well).
0069_Diagramm_B
Fig. B: Comparison of cell counting results using ROTITEST® Vital (10 μl/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM).


ROTITEST®Vital
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Order No. Pack Qty. Pack. Packaging Price Quantity
0069.1 5 ml plastic 1 x 5 ml €169.50
0069.2 20 ml plastic 4 x 5 ml €509.00
0069.3 1 ml plastic 1 x 1 ml €60.90
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Certificates of Analysis

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The following analysis certificates have been found:

Type analysis

Appearance (test solution)red  
Turbiditytransparent  
Absorptioncomplies  

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