Ammonium iron(III) citrate solution

12,5 %, sterile, ready-to-use
Media supplement for detection of Listeria monocytogenes.
One box of 10 vials Ammonium iron(III) citrate solution is sufficient for 10 x 500 ml Fraser Broth.
Ferric(III) ammonium citrate solution

Ammonium iron(III) citrate solution
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Storage temp.: +20 °C
WGK 1

Ammonium iron (III) citrate (ferric ammonium (III) citrate) is used as additive in nutrient media for detection of Listeria species. Hydrolysis of esculin by Listeria species results in 6,7-dihydroxycoumarin which reacts with ferric ions producing blackening of the medium. Additionally, ferric ammonium citrate has been shown to improve the growth of Listeria monocytogenes (Cowart and Foster (1985) J. Infect. Dis. 151:721).

Application: One vial ferric ammonium (III) citrate solution (0.25 g in 2 ml) is added to 500 ml autoclaved Fraser Broth (Art. No. HP17) or Fraser Half Broth (Art. No. HP18). Mix carefully and store cooled for 2 weeks in maximum. Fraser Broth is used in the rapid detection of Listeria from food and environmental samples (Fraser and Sperber (1988) J. Food Protect. 51:762).


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Ammonium iron(III) citrate solution

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock 3130.1 10 x 2 ml plastic €41.40 €32.10
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Item available in stock 3130.2 30 x 2 ml plastic €103.75 €80.85
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Technical information

Formulation per vial:
Ammonium iron(III) citrate0,25 g

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