HEPES

PUFFERAN® CELLPURE® ≥99,5 %
For cell culture, tissue culture and biotechnology.
N-2-Hydroxyethyl piperazine-N'-2-ethane sulphonic acid, Good's buffer

HEPES
  • 00035449_0.jpg
Empirical formula C8H18N2O4S
Molar mass (M) 238,31 g/mol
Melting point (mp)234-238 °C
WGK 3
CAS-Nr. [7365-45-9]
EG-Nr. 230-907-9

Extremely versatile buffer. Excellent for cell and tissue cultures and for analysis of biological systems.

Endotoxins: approx. 0.025 E.U./mg

Applications:

  • Combined with bicarbonate, HEPES is an excellent medium for human lymphocytoidal cell lines
  • HEPES is more effective than NaHCO3 when controlling the pH value in tissue and organ culture
  • HEPES-buffered media for chicken and human lung fibroblasts
  • Can be used to replace a bicarbonate buffered medium containing serum with a serum-free HEPES-buffered medium for mouse fibroblasts

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HEPES

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock HN77.1 50 g plastic €27.85 €21.70
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Item available in stock HN77.2 100 g plastic €40.75 €31.75
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Item available in stock HN77.3 250 g plastic €77.30 €60.25
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Item available in stock HN77.4 500 g plastic €137.50 €107.20
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Item available in stock HN77.5 1 kg plastic €237.05 €184.85
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Technical information

Guarantee analysis:
Assay (titr.)≥99,5 %
Water (KF)≤0,2 %
Chloride (Cl)≤0,005 %
Sulphate (SO4)≤0,01 %
Phosphate (PO4)≤0,001 %
Heavy metals (as Pb)≤0,0005 %
Arsenic (As)≤0,00005 %
Iron (Fe)≤0,0003 %
UV-absorption (1 M in H2O):
260 nm≤0,04
280 nm≤0,03
Endotoxinstested
DNases, RNases, Proteasenone detected

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