Roti®-Fect

CELLPURE® for transfection
Reagent for liposoma-mediated transfection of eukaryotic cells.
Approx. 400 assays per ml in 24wells. Dependent on the cell line used.

Roti<sup>®</sup>-Fect
  • 00098234_0.jpg
Storage temp.: +4 °C
WGK 1

  • Highly efficient reproduction of DNA in mammal cells
  • Excellent reproducibility
  • High and wide operating plateau
  • No inhibition through serum
  • Low cell toxicity
  • Successful with different cell lines
  • Excellent transfection results

Roti®-Fect is a liposome formulation of a polycationic lipid in combination with a neutral colipid. Compared to monocationic lipid formulations, Roti®-Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently. An efficient triple-cotransfection using Roti®-Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.

Successfully tested with the following cell types:

siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620
Primary cells: pAML, pHDF, pMEF, pRHC, pSMC
Cell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al.

Figure:

Transfection of a variety of cell lines with Roti®-Fect and other commercially available transfectants. Transfection was performed in 24wells using 0.25 µg DNA each, with 1.5 µl Roti®-Fect or acc. to instructions given by the manufacturers. Luciferase activity was measured 48 hours post transfection. Data of cells transfected with Roti®-Fect was set to 100 %.


Show more Show less

Roti®-Fect

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock P001.1 0,2 ml plastic €73.00 €58.35
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available in stock P001.2 0,5 ml plastic €163.30 €130.60
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available in stock P001.3 1 ml plastic €255.75 €204.55
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available at short notice P001.4 5 x 1 ml plastic €1,106.20 €884.70
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available in stock Item available at short notice Item not available
Add to shopping basket

Download links

CoAs

You can search and download here your CoA. Please enter the corresponding item number and batch number.

Search results

Following CoAs have been found:

Error

    Search CoA
    Demand CoA

    Fields marked * are mandatory.

    Request placed

    Your request of generating a CoA has been successfully completed.

    Choose Market

    Please choose your market and your language.

    save settings