Roti®-Pol TaqS Mix (2x)

ready-to-use, 2x conc.
Taq master mix for all standard PCR amplifications.
Optimized for reactions of 25 µl volume.
Master mix Taq DNA polymerase, recombinant

Roti<sup>®</sup>-Pol TaqS Mix (2x)
  • 00034438_0.jpg
Storage temp.: -20 °C
Transport temp.: cooled
WGK 1

Optimized pre-mixed 2x PCR solution containing the recombinant TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2, and all other components required for PCR except primers and template DNA. Roti®-Pol TaqS Mix (2x) is recommended for use in all standard PCR applications.

PCR assaying with Roti®-Pol TaqS Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. Roti®-Pol TaqS Mix (2x) can be applied to all standard Taq-based cycling protocols and is, therefore, the optimal choice for high throughput PCR as being performed in, for instance, analysis of cloning efficiency or colony screening, for routine screening processes, and for student's courses.

Due to the optimized composition of the master mix, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates. Roti®-Pol TaqS Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. The use of the colourless PCR Master mix is particularly recommended when direct fluorescence or absorbance readings are required. The TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.

For research use only. Not approved for use in clinical or in vitro diagnostics.

Compositon:

2 or 10 vials, respectively, with 1,25 ml each of Roti®-Pol TaqS Mix (2x) containing TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer.


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Roti®-Pol TaqS Mix (2x)

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock 9239.1 2,5 ml plastic €43.00
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Item available in stock 9239.2 12,5 ml plastic €172.00
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Technical information

Type analysis:
Appearancecolourless
Endonuclease activitynone detected
Exonuclease activitynone detected
Suitability for PCR (400 bp)complies
Suitability for PCR (3 kb)complies
Selective specificity in PCRcomplies
For research use only. Not approved for use in clinical or in vitro diagnostics.

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