4x concentrated SDS-PAGE matrix buffer, for electrophoresis
Ready-made buffer solution for pouring of optimized SDS-PAGE gels.
One bottle of 250 ml is sufficient for pouring of approx. 100 mini gels.
SDS-Page buffer, Gel matrix buffer
Rotiphorese®-PAGE Matrix Buffer plus is a 4x concentrated gel matrix buffer for enhanced protein separation and significantly higher gel strenth.
Rotiphorese®-PAGE Matrix Buffer plus can be used for preparation of both stacking gel and resolving gel by simply replacing the Laemmli/TRIS-, or TRICIN-, buffer system. Even gels with stacking gels of only one acrylamide concentration, show a protein separation quality comparable to that of gradient gels. Band depiction is significantly enhanced compared to standard buffer systems.
Rotiphorese®-PAGE Matrix Buffer plus also provides increased gel strength, making handling of the gels much more convenient, for instance during separation of the glass plates or during transport. Gel stability is increased, leading to longer shelf lifes of self-made gels. Gels prepared with Rotiphorese®-PAGE Matrix Buffer plus may also very well be used for 2D protein analysis or for subsequent Western Blotting onto NC or PVDF membranes.
Figure 1 and 2:
Coomassie® stained gel and Western blot. HeLa extract on PVDF, detected via HRP chemoluminescence.
A: 10 % PAGE using Rotiphorese®-PAGE Matrix buffer plus,
B: 12 % PAGE using standard Tris glycine SDS buffer.
Silver staining. HL-60 cell extract after 1D-IEF (pH 4-7) and 2D gel electrophoresis in a 12 % PA gel using Rotiphorese®-PAGE Matrix buffer plus.
Stability of a 6 % PA gel.
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