Rotitest® Vital

Rotitest® Bio Analysis
Colorimetric test solution for evaluation of cell proliferation and -viability.
1 ml is sufficient for enumeration of 100 cell samples.
Non-cytotoxic cell proliferation test solution.

Rotitest<sup>®</sup> Vital
  • 00035425_0.jpg
  • 00035489_0.jpg
Storage temp.: +4 °C
WGK 2

  • Perfectly supersedes MTT
  • Cells keep on being vital
  • Most simple handling
  • One-component-system without radioactivity
  • For adherent and suspension cells
  • Photometric result in 1 to 4 hours
  • Strong correlation between absorbance and cell number
  • Suitable for every culture media

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.

In all living cells, NADH and NADPH are formed by the respiratory chain. Roti®-Test vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.

The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm). Also suitable for culture media containing phenol red.

Example of use.

Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [3H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al., Anal. Commun. 1999 (36) 47-50).

Fig. A: Correlation of cell counting results achieved with Rotitest® Vital (10 µl/well) and 3H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM).

Fig. B: Comparison of cell counting results using Rotitest® Vital (10 µl/well) and other cell counting reagents. Photometrical measurement at 450 nm (Rotitest® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM).

Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with Rotitest® Vital (10 µl/well).

Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using Rotitest® Vital (10 µl/well).

Each package contains simple-to-follow instructions-for-use.

Stabile at ambient temperature. Very stabile when being cooled.

Store at +4 °C protected from light. Do not freeze.


Show more Show less

Rotitest® Vital

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock 0069.3 1 ml plastic €57.95 €45.15
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available in stock 0069.1 5 ml plastic €166.65 €129.95
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available in stock 0069.2 4 x 5 ml plastic €504.20 €393.20
Add to shopping basket
Close

Item added to your shopping basket.

Please insert a valid amount.

Item available in stock Item available at short notice Item not available
Add to shopping basket

Technical information

Type analysis:
Appearance (test solution)red
Turbiditytransparent
Absorptioncomplies

CoAs

You can search and download here your CoA. Please enter the corresponding item number and batch number.

Search results

Following CoAs have been found:

Error

    Search CoA
    Demand CoA

    Fields marked * are mandatory.

    Request placed

    Your request of generating a CoA has been successfully completed.

    Choose Market

    Please choose your market and your language.

    save settings