X-β-Gal

≥99 %, BioScience-Grade
Colorimetric substrate of β-galactosidase. For biochemistry and molecular biology.
5-Bromo-4-chloro-3-indoxyl-β-D-galactoside

X-β-Gal
  • 00100214_0.jpg
Empirical formula C14H15BrClNO6
Molar mass (M) 408,6 g/mol
Melting point (mp)225-230 °C
Storage temp.: -20 °C
Transport temp.: room temp.
WGK 1
CAS No.[7240-90-6]
EG-Nr. 230-640-8

Ideal for colorimetric detection of β-galactosidase activity, for instance during blue-/white selection.

Application:

Stock solution: 100 mg/ml (240 mM) in DMF, store at -20 °C in the dark. Working solution: For blue/white selection of recombinant clones on agar plates use 0,3 µl stock solution per ml broth/agar.


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X-β-Gal

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock 2315.1 100 mg glass €21.40 €17.05
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Item available in stock 2315.2 500 mg glass €61.20 €48.90
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Item available in stock 2315.3 1 g glass €114.50 €91.55
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Item available in stock 2315.4 5 g glass €385.95 €308.40
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Technical information

Guarantee analysis:
Appearancewhite to off-white powder
Assay (HPLC)≥99 %
Specific rotation [α]20/D (c=1 in DMF/H2O 1:1)-62° ±2°
Water (KF)≤0,2 %
DNases, RNases, Proteasenone detected

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