Ribonuclease A

90 U/mg (Kunitz), BioScience-Grade, free from salt
Acc. to Hirs, extracted from bovine pancreas.
RNase A

Ribonuclease A
  • 00069815_0.jpg
Molar mass (M) ca. 13 700 g/mol
Storage temp.: -20 °C
Transport temp.: room temp.
WGK 1
CAS No.[9001-99-4]
EG-Nr. 232-646-6

Extracted from bovine pancreas for RNA separation, especially when isolating RNAfree DNA. The enzyme cleaves RNA and produces 3' terminal nucleoside phosphates. This quality product for molecular biology is free from salt, without proteases and is chromatographically homogeneous. Unit definition acc. to Kunitz.

DNAse content of the RNAse powder ist below detection limit. However, we nevertheless recommend pretreatment of the ribonuclease prior to using it for DNA isolation. DNAse free RNAse ist prepared by cooking the water-solubilised ribonuclease aliquots for 15 min. Let cool to room temperature on bench top.

Application:

Stock solution: 10 mg/ml in water (pH ≤6,0) or 0,01 M sodium acetate (pH 5,2). Do not heat RNAse prepared in sodium acetate.

Working solution: 2-10 µg/ml in 10 mM Tris (pH 7,5), 15 mM NaCl.

Note: RNAse precipitates when concentrated solutions are heated/cooked at pH ≥7,0.

Store in aliquots at -20 °C.


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Ribonuclease A

Status Art. No. Pack Qty. Packaging Price Quantity Action
Item available in stock 7156.1 100 mg glass €72.60 €58.05
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Item available in stock 7156.2 500 mg glass €321.45 €256.80
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Item available at short notice 7156.3 1 g glass €525.70 €420.20
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Technical information

Type analysis:
Appearancewhite, freeze-dried powder
Assay (IEC, Amberlit CG50)>95 %
Solubility (2 mg/ml, H2O)clear, soluble
Activity (Kunitz)ca. 90 U/mg
DNAsenone detected
Proteasenone detected

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