User manual DE
For purification and concentration of biomolecules and for purification of solutions.
Centrifugal devices for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates.
Roti®-Spin Centrifugal Devices can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Centrifugation can easily be performed in the cold room or at specially designated benches like isotope laboratories or in laminar flow hoods.
For convenient and successful application, centrifugal devices with membranes in 4 colour-coded molecular weight cut-offs (MWCO) are available. In order to choose the appropriate MWCO please use the following thumb-rule:
Centrifugation of proteins/peptides: MWCO ≤1/3 of sample molecule size.
Centrifugation of nucleic acids: MWCO ≤ ½ sample molecule size.
Overview for selecting the right pore size
|MWCO||Molec. size||DNA size|
|3 kD||10 - 20 kD||<50 bp|
|10 kD||30 - 90 kD||50 - 200 bp|
|30 kD||90 - 180 kD||200 bp - 1 kb|
|100 kD||>300 kD||>1 kb|
Maximum sample volume: Roti®-Spin MINI 50-500 µl
Final concentrate volume: Roti®-Spin MINI 15-20 µl
Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC.
|Status||Art. No.||Type||MWCO (kD)||Colour||Pack Qty.||Price||Quantity||Action|
|Item available in stock||CL12.1||Roti®-Spin MINI-3||3||grey||25||€111.80|
|Item available in stock||CL13.1||Roti®-Spin MINI-10||10||blue||25||€111.80|
|Item available at short notice||CL14.1||Roti®-Spin MINI-30||30||red||25||€111.80|
|Item available at short notice||CL15.1||Roti®-Spin MINI-100||100||clear||25||€111.80|
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