Roti®-Spin MINI

Roti<sup>®</sup>-Spin MINI

For purification and concentration of biomolecules and for purification of solutions.

  • Applicable for nucleic acids, proteins/peptides, other biomolecules >6 kD and very small bioparticles
  • Can be used to replace standard techniques like precipitation, dialysis, gel filtration, gel purification, column chromatography, gradient centrifugation etc.
  • Gentle: No shearing of DNA of up to 100 kb, proteins remain native and enzymatically active
  • Applicable for purification of all labelled probes (isotope-, fluorescent-, chromogenic labelling)
  • Very high recovery rates of >90 % (when using devices of appropriate MWCO)

Centrifugal devices for ultrafiltration of biomolecules. The membranes made of highly chemical-resistant polyethersulfone have been tightly sealed to prevent leakage and loss of samples. Furthermore, the membranes underwent special treatment minimising the binding of biomolecules, in order to optimise sample recovery rates.

Roti®-Spin Centrifugal Devices can be applied to a variety of applications and can be used to replace tedious and labour-intensive methods like gel filtration, gel purification or precipitation. Application is time-saving and very simple - only few centrifugal steps are necessary to concentrate and recover your sample. During this, the sample remains untouched in the upper reservoir, and cross-contamination is efficiently prevented even when large numbers of samples are handled. Centrifugation can easily be performed in the cold room or at specially designated benches like isotope laboratories or in laminar flow hoods.

MWCO:
For convenient and successful application, centrifugal devices with membranes in 4 colour-coded molecular weight cut-offs (MWCO) are available. In order to choose the appropriate MWCO please use the following thumb-rule:
Centrifugation of proteins/peptides: MWCO ≤1/3 of sample molecule size.
Centrifugation of nucleic acids: MWCO ≤ ½ sample molecule size.

Overview for selecting the right pore size

MWCOMolec. sizeDNA size
3 kD10 - 20 kD<50 bp
10 kD30 - 90 kD50 - 200 bp
30 kD90 - 180 kD200 bp - 1 kb
100 kD>300 kD>1 kb

Sample volumes:

Maximum sample volume: Roti®-Spin MINI 50-500 µl
Final concentrate volume: Roti®-Spin MINI 15-20 µl

Applications (examples):

Desalting or buffer exchange following restriction digest, PCR or such; separation of free nucleotides and polymerised DNA; removal of contaminating compounds (like DNA) from stock solutions (e.g. PCR-buffers); concentration of DNA or proteins; separation of proteins of different size; sample preparation prior to HPLC.

Roti®-Spin MINI


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Roti®-Spin MINI

Status Art. No. Type MWCO (kD) Colour Pack Qty. Price Quantity Action
Item available in stock CL12.1 Roti®-Spin MINI-3 3 grey 25 €111.80
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Item available in stock CL13.1 Roti®-Spin MINI-10 10 blue 25 €111.80
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Item available at short notice CL14.1 Roti®-Spin MINI-30 30 red 25 €111.80
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Item available at short notice CL15.1 Roti®-Spin MINI-100 100 clear 25 €111.80
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