Roti®-Pol DNA Polymerases – Overview

Roti<sup>®</sup>-Pol DNA Polymerases – Overview

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus or Pyrococcus furiosus.

Roti®-Pol, the new series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the Roti®-Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

In addition to the standard Taq polymerase for PCR assays in general, the range also includes the antibody blocked hot-start version of the enzyme, which is recommended for particularly sequence specific approaches. This inhibited Taq polymerase is getting active only after the initial denaturation step, resulting in amplification of the target sequence without production of unwanted side products. Both Taq polymerases are available as set of enzyme and buffer solutions as well as premixed, 2x concentrated master mixes. The sets contain the DNA polymerase plus two 10x concentrated reaction buffers with MgCl2, one of which has been specially designed for direct gel loading following the PCR reaction. Besides the DNA polymerase, the master mixes include dNTPs, MgCl2, and all other components required for PCR except primers and template DNA, and are available in a colourless version for PCR only, plus a red ready-to-load version for subsequent direct gel electrophoresis.

With the TaqHY DNA polymerase we provide a modified Taq polymerase outstanding in robust performance, fast polymerisation rate and high yield of amplicons. Combining the benefits of both techniques, the Hot-TaqHY DNA polymerase has been designed by increasing the PCR sequence specificity of a quick and high yield modified Taq polymerase by antibody mediated inhibition.

In combination with the attached specially adjusted high fidelity buffer, the ProofRead polymerase delivers highly sequence-true PCR amplicons and is recommended for amplification of target sequences of high GC content, or with complex secondary structures.

All TaqUltra DNA polymerase are being tested on absence of DNA (bacterial genomic DNA) which makes them particularly suitable for amplification of bacterial DNA. This polymerase is also available in standard version as well as the hot-start type Hot-TaqUltra.

Roti®-Pol DNA polymerases are able to amplify PCR products up to 5 kb with genomic, viral, cDNA and plasmid DNA as template. The Taq DNA polymerases possess a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, generating amplicons with a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes. In addition to the 5’ → 3’ polymerase activity, the Pfu DNA polymerase possesses a 3’ → 5’ (proof reading) exonuclease activity, rapidly substituting misincorporated bases during polymerization. These DNA fragments are blunt-ended.

For easy handling, all solutions are filled in tubes with colour coded lids. The red dye for direct gel loading, included in buffers and master mixes, migrates approx. as fast as a 1 kb DNA fragment in 1 % agarose gels.

For research use only. Not approved for use in clinical or in vitro diagnostics.

Storage temp.: -20 °C • Transport temp.: cooled


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Roti®-Pol DNA Polymerases – Overview

Status Art. No. Product Purity Application Amplicon Ends Pack Qty. Price Quantity Action
Item available in stock 9350.1 Roti®-Pol Hot-TaqUltra 5 U/µl, DNA-free Particularly sequence specific PCR of bacterial gDNA 3'dA 200 U €149.45
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Item available in stock 9350.2 Roti®-Pol Hot-TaqUltra 5 U/µl, DNA-free Particularly sequence specific PCR of bacterial gDNA 3'dA 1000 U €733.15
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Item available in stock 9346.1 Roti®-Pol Hot-TaqHY 5 U/µl Particularly sequence specific, fast PCR protocols and/or high yield, GC rich template DNA 3'dA 200 U €67.65
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Item available in stock 9346.2 Roti®-Pol Hot-TaqHY 5 U/µl Particularly sequence specific, fast PCR protocols and/or high yield, GC rich template DNA 3'dA 1000 U €320.35
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Item available in stock 9245.1 Roti®-Pol Hot-TaqS 5 U/µl Particularly sequence specific standard PCR 3'dA 200 U €60.20
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Item available in stock 9245.2 Roti®-Pol Hot-TaqS 5 U/µl Particularly sequence specific standard PCR 3'dA 1000 U €287.05
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Item available in stock 9248.1 Roti®-Pol Hot-TaqS Mix (2x) ready-to-use, 2x conc. Particularly sequence specific standard PCR 3'dA 2,5 ml €64.50 €48.35
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Item available in stock 9248.2 Roti®-Pol Hot-TaqS Mix (2x) ready-to-use, 2x conc. Particularly sequence specific standard PCR 3'dA 12,5 ml €301.00 €225.75
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Item available in stock 9256.1 Roti®-Pol Hot-TaqS Red-Mix (2x) ready-to-use, 2x conc. Particularly sequence specific standard PCR with subsequent gel loading 3'dA 2,5 ml €68.80 €51.60
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Item available in stock 9256.2 Roti®-Pol Hot-TaqS Red-Mix (2x) ready-to-use, 2x conc. Particularly sequence specific standard PCR with subsequent gel loading 3'dA 12,5 ml €318.20 €238.65
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Item available in stock 9344.1 Roti®-Pol ProofRead 5 U/µl High fidelity PCR with exact sequence replication blunt 200 U €77.40
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Item available in stock 9344.2 Roti®-Pol ProofRead 5 U/µl High fidelity PCR with exact sequence replication blunt 1000 U €365.50
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Item available in stock 9345.1 Roti®-Pol TaqHY 5 U/µl Fast PCR protocols and/or high yield, GC rich template DNA 3'dA 500 U €66.55
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Item available in stock 9345.2 Roti®-Pol TaqHY 5 U/µl Fast PCR protocols and/or high yield, GC rich template DNA 3'dA 2500 U €318.20
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Item available in stock 9223.1 Roti®-Pol TaqS 5 U/µl Standard PCR 3'dA 500 U €60.10
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Item available in stock 9223.2 Roti®-Pol TaqS 5 U/µl Standard PCR 3'dA 2500 U €284.90
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Item available in stock 9239.1 Roti®-Pol TaqS Mix (2x) ready-to-use, 2x conc. Standard PCR 3'dA 2,5 ml €43.00 €32.25
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Item available in stock 9239.2 Roti®-Pol TaqS Mix (2x) ready-to-use, 2x conc. Standard PCR 3'dA 12,5 ml €172.00 €129.00
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Item available in stock 9241.1 Roti®-Pol TaqS Red-Mix (2x) ready-to-use, 2x conc. Standard PCR with subsequent gel loading 3'dA 2,5 ml €47.30 €35.45
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Item available in stock 9241.2 Roti®-Pol TaqS Red-Mix (2x) ready-to-use, 2x conc. Standard PCR with subsequent gel loading 3'dA 12,5 ml €186.00 €139.45
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Item available in stock 9347.1 Roti®-Pol TaqUltra 5 U/µl, DNA-free PCR of bacterial gDNA 3'dA 200 U €143.00
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Item available in stock 9347.2 Roti®-Pol TaqUltra 5 U/µl, DNA-free PCR of bacterial gDNA 3'dA 1000 U €698.75
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