Technical Data Sheet
RNA-ClickSeq Library Prep Kit
The products marked accordingly are shipped refrigerated in special, sustainable cooling boxes with cooling packs or dry ice.
Transport temp. cooled
€564.40/Pack Qty.
excl. VAT. | 1 kit per Pack Qty.
Art. No. 3L83.1
Product details
RNA-ClickSeq Library Prep Kit
ClickSeq is a simple method for synthesizing NGS libraries using click-chemistry instead of traditional ligation enzymes to attach sequencing adapters—a step often prone to errors. During reverse transcription, 3′-azido-nucleotides are incorporated, which terminate the growing cDNA strands. These azido-blocked cDNAs are then click-ligated to alkyne-modified adapters and PCR-amplified to generate sequencing-ready libraries.ClickSeq is a flexible and modular platform that supports both RNA and DNA inputs. By avoiding enzymatic ligation and fragmentation, the protocol is faster and more efficient than standard RNA-seq workflows.
- Click Primer Mix
- Click Mix
- Click Accelerant
- Click Catalyst
- i7 Index Primers (Dxx-Dxx)
- Elution Buffer 1
- Elution Buffer 2
- No enzymatic ligation required
- No RNA fragmentation needed
- Faster and simpler workflow
- Ultra-low chimera rate (only 3 per million reads)
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| Art. No. | Pack Qty. | Pack. | Packaging | Price | Quantity | |
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| 3L83.1 | 1 kit | cardboard | 12 reactions |
€564.40 |
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Downloads / MSDS
General information
The copper(I)-catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1-3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
In recent years, click chemistry has become increasingly important and steadily more popular in research and development. The reasons for this are not only the easy handling and the high yields but also the countless application possibilities. Carl ROTH offers you the possibility to purchase the broad portfolio of click reagents, various kits for EdU-based cell proliferation assays and numerous other applications from the well-known brand baseclick. This enables us to offer you a necessary and comprehensive assortment for click chemistry.