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Membrane de transfert ROTI®Nylon 0.45, 15 × 15 cm
118,25 €/cdt.
TVA en sus. | 5 feuille(s) par cdt.
Réf. 260Y.1
Convient à / Accessoires
Données du produit
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Membrane de transfert ROTI®Nylon 0.45
The optimal membrane for DNA/RNA transfer, especially colony and plaque lifting and all multiple rehybridisations.
ROTI®Nylon 0.45 is a neutral, reinforced nylon membrane. Like all ROTI®ylon membranes, it has a very high tensile strength and is highly resistant to alcohols, acetone and many other organic solvents.
ROTI®Nylon 0.45 is not charged at buffer pH 6.5 and has excellent hydrophilic properties due to the amino and carboxy groups on the surface. The membrane charge (ζ-potential) can be adjusted by the pH value of the buffer system (positive membrane charge at pH<6.5; negative membrane charge at pH>6.5). Steam sterilisation is possible.
The covalent binding of transferred nucleic acid is achieved by crosslinking (UV crosslinking for 20 s at 260 nm). The excellent signal/background ratio of the membrane then enables highly sensitive detections (single copy gene detection) and multiple rehybridisations without loss of signal quality.
Detection by radioactivity, chemiluminescence, fluorescence
- Southern et Northern blotting
- Western blotting
- Microarrays et Macroarrays
- Dot/Slot blotting
Dimensions | 15 × 15 cm |
Taille de pores | 0,45 µm |
Liaison | hydrophile |
Charge | neutre/amphotére |
Capacité de liaison | 350 µg/cm² |
Épaisseur | 140-190 µm |
Immobilisation | UV |
- Somme intermédiaire : 0.00
Réf. | Dimensions | Cdt. | Prix | Quantité | |
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260P.1 | 300 × 30 cm | 1 rouleau(x) |
456,90 € |
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260T.1 | 300 × 15 cm | 1 rouleau(x) |
236,50 € |
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260X.1 | 20 × 20 cm | 5 feuille(s) |
166,65 € |
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260Y.1 | 15 × 15 cm | 5 feuille(s) |
118,25 € |
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- Somme intermédiaire : 0.00