Agarose High Resolution, 500 g
CAS No. [9012-36-6]
EG-Nr. 232-731-8
€1,607.15/Pack Qty.
excl. VAT. | 500 g per Pack Qty.
Art. No. K297.3
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Product details
Our agarose ROTI®Garose is a neutral polysaccharide which is obtained in many purification steps from the cell wall of certain algae, called Rhodophyceae. ROTI®Garose forms very clear gels with all standard running buffers and will result in a sharp and clear separation of your biomolecules. Extremely pure agarose with very low interference binding to staining reagents. ROTI®Garose produces a low background and high contrast appearance after staining.
- For clear and sharp bands
- Gels with high transparency
- Low background
- Suitable for all standard running buffers and high-speed buffer systems
- Compatible with all nucleic acid staining systems
- Non-toxic in cell immobilisation assays
- Of course DNase and RNase free
Chemically, agarose is a galactan which is capable of forming extremely solid gels, even at a low concentration. The pore size of the gels is determined by the concentration of the agarose used. Because there are no ionic groups in the gel, hydrophilic materials without any interaction with the gel matrix will also be separated according to their size. The high hysteresis of the agarose, that is the thermal stability after solidification, ensures that the gels remain reliably stable even during heatproducing running conditions.
Agarose High Resolution ROTI®Garose for small fragments
- High resolution of PCR-products and DNA fragments in the 50-1000 bp range
- Low gel background after staining, even for highly concentrated gels
- Can be used in concentrations of up to 5 % agarose
- Low DNA binding and low EEO for efficient separation of very small fragments
- Tip: cool the gel for 30 mins. at 4-8 °C before use for superior separation
- Separation range in 1 x TAE:
1,8 % proof agarose gel: 500-1000 bp
3,0 % proof agarose gel: 150-600 bp
4,5 % proof agarose gel: 50-350 bp
| Application | Analysis of fragments between 50 and 1000 bp |
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| K297.1 | 10 g | plastic | €57.95 |
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| K297.2 | 100 g | plastic | €375.20 |
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| K297.3 | 500 g | plastic | €1,607.15 |
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- Subtotal: 0.00
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Certificates of Analysis
Guarantee analysis
| Electroendosmosis (EEO) | ≤0,12 |
| Water content | ≤7 % |
| Ash | ≤0,35 % |
| Sulphate (SO4) | ≤0,11 % |
| Gel strength (1,5 % gel) | ≥600 g/cm2 |
| Gel strength (3 % gel) | ≥1500 g/cm2 |
| Gelling temperature (3 % gel) | ≤35,5 °C |
| Melting temperature (3 % gel) | ≤80 °C |
| Turbidity (1,5 % solution) | ≤5 NTU |
| DNases, RNases | none detected |