Denhardt's solution, lyophilised powder (50x)
The bottle contains powder mix for 50 ml 50x stock solution.Add 48,8 ml water to gain exactly 50 ml stock solution. Sterilise stock solution by filtration (0,2 µm) and store in aliquots of 10 ml at –20 °C .
excl. VAT. | 50 ml per Pack Qty.
Art. No. HP33.1
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Denhardt's solution, lyophilised powder (50x) BioScience Grade, for molecular biology
Denhardt's lyophilised powder contains a mixture of different blocking agents that is used for nuclear acid hybridisation (Northern and Southern Blot) as well as for stripping of hybridised membranes. By prehybridisation the filter membranes with Denhardt's solution unspecific binding of DNA to the membrane is avoided.
For hybridisation use 10x Denhardt's solution with 6x SSC (ROTI®Stock 20x SSC, Art. No. 1054.1) supplemented with 100 µg/ml denatured salmon sperm DNA and 1 % SDS (ROTI®Stock 20 % SDS, Art. No. 1057.1).
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|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|HP33.1||50 ml||plastic||Powder for 50 ml||€103.75||
Delivery date currently unknown
Delivery time on request
|Albumin Fraction V||0,5 g|
|Ficoll 400||0,5 g|
|Polyvinylpyrrolidone K30||0,5 g|
|pH-value (stock solution in water)||6,3 ±0,2|