Lambda Hind III / phiX Hae III Marker
Transport temp. ambient temp.
Number of fragments: 17
Number of lanes per 100 μg: approx. 200 (mini gels)
DNA ladder in lyophilised form. Gel loading buffer (Art. No. 0100.1) for dissolving the DNA and subsequent application on gels is supplied.
Manufactured by rest ...
excl. VAT. | 50 µg per Pack Qty.
Art. No. CP49.1
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Lambda Hind III / phiX Hae III Marker DNA marker + gel loading buffer, not pre-dyed
Versatile marker for depiction of all standard DNA fragment lengths.
Manufactured by restricting unmethylated DNA with the restriction endonucleases Hind III and Hae III.
By mixing high molecular weight Lambda (Hind III) fragments with short-sequence phiX-174 (Hae III) fragments a marker was designed which covers a broad range from 200 bp up to 20 kb. Most fragments are distributed in an equimolar way, additionally allowing rough DNA quantification in the gel. Due to this equimolarity, however, the smallest bands of 125 bp, 118 bp and 72 bp can not be detected efficiently in standard gels.
Optimal results are obtained with a running distance of approx. 10 cm.
Fig.: 1 % agarose (NEEO, Art. No. 2267).
|Number of fragments||17|
|Number of lanes||~200/100 µg|
|Size range||72–23130 ppt|
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