Jump to content Jump to navigation menu

ROTI®Pol Hot-TaqUltra, 40 µl, 1 x 200 U

5 U/μl, DNA-free
Icon_Eisstern All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Pack Qty.
DNA polymerase (thermostable), Hot start DNA polymerase, Taq DNA polymerase, recombinant, Polymerase (thermostable)
Storage temp. -20 °C
Transport temp. cooled

For hot start PCR amplifications, particularly of bacterial DNA.
Ultra pure HotTaq polymerase in storage buffer

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticu ...
Product details
Type analysis

€160.75/Pack Qty. 

excl. VAT. | 40 µl per Pack Qty.

Art. No. 9350.1

Delivery date currently unknown
Free of shipping costs from 125 €
from 6 Pack Qty. €152.71/Pack Qty.
from 24 Pack Qty. €144.68/Pack Qty.
Carl Roth subscription service

Now recurring orders conveniently delivered as a subscription!

With the new Carl Roth Replenishment Service you can let products be ordered automatically which you need regularly in your lab!

How it works:

Put all products for your subscription in the desired quantity in the basket.


In the shopping cart, select the option "Order shopping cart as subscription" Order as subscription.


Set Starty point and interval for your subscription and submit order!

By the way: Through your account you can customize or delete your subscriptions anytime.

Any questions about this product?

Do you have a question about this product?

In the general information or Product details, we have compiled extensive descriptions and downloads for each product.
If you can not find an answer to your question in the Certificates of Analysis and Application Examples , feel free to contact us!
Contact Service

Product details

For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.

ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.

ROTI®Pol Hot-TaqUltra 5 U/μl, DNA-free

Hot start version of the recombinant heat stable Taq DNA polymerase from the thermophilic bacterium Thermus aquaticus in storage buffer and tested on the absence of bacterial DNA. ROTI®Pol Hot-TaqUltra is recommended for use in highly specific PCR applications in general, or if bacterial DNA, or in RT-PCR, bacterial 16S rRNA shall be detected.
ROTI®Pol Hot-TaqUltra is purified using a multiple-step process that minimizes contaminating bacterial DNA to a none detectable level. Each lot of the polymerase undergoes strict quality control testing in order to ensure the absence of detectable amounts of contaminating bacterial DNA. ROTI®Pol Hot-TaqUltra is provided in a DNA-free storage buffer containing 50 % glycerol.
ROTI®Pol Hot-TaqUltra is recommended for use when particularly specific amplification of bacterial DNA is the main focus. The antibody-mediated blocking of the DNA polymerase is released only at the initial denaturation step, hence resulting in highly specific amplification of the target sequence without production of unwanted side products caused by unspecific primer annealing.
ROTI®Pol Hot-TaqUltra is able to amplify PCR products up to 5 kb and is appropriate for use in the amplification of DNA from eukaryotic as well as prokaryotic templates. The Hot-TaqUltra DNA polymerase possesses a 5’ → 3’ polymerase- as well as a 5’-3' exonuclease activity, and generates a 3'dA (adenine)-overhang which may well be used for TA-cloning purposes.

Technical Information
Use Particularly sequence specific PCR of bacterial gDNA  
Amplicon ends 3'dA  
Polymerase DNA free, ultra pure hot start Taq polymerase  
Use Particularly sequence specific PCR of bacterial gDNA  
ROTI®Pol Hot-TaqUltra
Selected quantity:   0
  1. Subtotal:  0.00
Order No. Pack Qty. Pack. Packaging Price Quantity
9350.1 40 µl plastic 1 x 200 U €160.75
9350.2 200 µl plastic 5 x 200 U €768.65
In stock
Delivery date currently unknown
Not available
Delivery time on request
Selected quantity:   0
  1. Subtotal:  0.00


Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Purity (enzyme)≥98%  
Enzyme activity5 U/µl  
Endonuclease activitynone detected  
Exonuclease activitynone detected  
Suitability for PCR (400 bp)complies  
Suitability for PCR (3 kb)complies  
Bacterial DNA (enzyme)none detected  
Selective specificity in PCRcomplies  
Hot start functionalitycomplies  
Stability (in storage buffer)complies  
For research use only. Not approved for use in clinical or in vitro diagnostics.