Biotin-11-dUTP, 25 µl, 25 nmol
Molar mass (M) 859,65 g/mol
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. cooled
excl. VAT. | 25 µl per Pack Qty.
Art. No. 1047.1
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- DNAse-, RNAse-, Protease- and Phosphatase-free
- Free of PCR inhibitors like modified bases and tetra-pyrophosphate
- Adjusted to pH 7,5 for optimzed enzyme compatibility
- Highly efficient enzymatic fabrication
- Can be optimally combined with Carl ROTH ROTI®Pol DNA polymerases
PCR, cDNA synthesis, labelling and primer-extension, mutagenesis-assays, sequencing reactions and in vitro transcription.
All Carl ROTH nucleotides are manufactured from highest-quality reagents and are most thoroughly tested for quality. This testing procedure not only includes standard-PCR but also ’long range PCR’, repeated quantitative light-cycling reactions, and tests for physical stability.
Biotin-11-dUTP ≥95 %, 1 mM solution
Biotin-11-dUTP is able to replace dTTP in growing DNA-strands and is used for efficient non-radioactive DNA-labelling. As a general rule, the labelled DNA is then detected using streptavidin-conjugates by colorimetric (e.g. streptavidin-AP) or fluorometric (e.g. fluorescein-streptavidin) assays.
Biotin-11-dUTP can be used for double staining techniques in combination with rhodamine-12-dUTP or fluorescein-12-dUTP and corresponding antibodies.
Applications: Non-radioactive labelling of DNA by enzymatic reactions, e.g. PCR, reverse transcription, nick-end-translation, end-labelling or random-primed DNA-labelling. Incorporation can be done with all established DNA-polymerases (e.g. Taq-polymerase, T4 DNA-polymerase, Klenow fragment).
Testet for the lack of endo-, exodeoxyribonuclease, ribonuclease and phosphatase.
ε240 = 10,7 E x mmol–1 x cm–1; ε289 = 7,1 E x mmol–1 x cm–1; pH: 7,5 ±0,2
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|1047.1||25 µl||plastic||25 nmol||€125.25||
|1047.2||50 µl||plastic||50 nmol||€210.60||
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