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Proteinase K, 500 mg, 1 x 500 mg

≥30 mAnson U/mg, lyophilized
Icon_DNAse-free
Icon_RNAse-free
Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Examples of effect: Have an allergenic effect, carcinogenic, mutagenic, toxic to reproduction and cause developmental toxicity (reprotoxic) or damage to organs Safety: You must be well-informed before starting work with these substances; wear protective clothing and gloves, eye protection and mask or breathing protection.
danger
H315-H317-H319-H334-H335
i causes skin irritation, may cause an allergic skin reaction, causes serious eye irritation, may cause allergy or asthma symptoms or breathing difficulties if inhaled, may cause respiratory irritation
P260.7 P280.7 P342+P311.0
i do not breathe dust, wear protective gloves/eye protection, if experiencing respiratory symptoms: Call a POISON CENTER/doctor
Pack Qty.
Pack.
Packaging
Storage temp. +4 °C
WGK 1
CAS No. [39450-01-6]
EG-Nr. 254-457-8

Non-specific protease for degrading proteins in biological samples. Isolated from fungi (Tritirachium album).
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.

Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease fami ...
Product details
Guarantee analysis

€317.15/Pack Qty. 

excl. VAT. | 500 mg per Pack Qty.

Art. No. 7528.2

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Product details


Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.


Directions for use

Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5.
pH optimum: 8,0. Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.


Working concentration

Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml



Proteinase K ≥30 mAnson U/mg, lyophilized

A non-specific protease with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.

Application examples:
  • Preparing chromosomal DNA for PFGE
  • Degrading nucleases during preparation of nucleic acids (includes preparation of native RNA)

Art. No. 7528.3: A stock solution with a concentration of 20 mg/ml can be prepared directly by adding 25 ml buffer or water.



Proteinase K
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Order No. Pack Qty. Pack. Packaging Price Quantity
7528.1 100 mg glass 1 x 100 mg €93.45
7528.2 500 mg glass 1 x 500 mg €317.15
7528.3 500 mg glass for direct preparation of stock sol. €353.70
7528.4 1 g glass 1 x 1 g €532.15
7528.5 250 mg glass 1 x 250 mg €177.40
7528.6 5 g glass 1 x 5 g €2,105.95
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Guarantee analysis

Activity (Hemoglobin, 37 °C)≥30 U/mg
DNases, RNasesnot detected
Endonucleasesnot detected

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