Proteinase K, 500 mg, 1 x 500 mg
CAS No. [39450-01-6]
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.
excl. VAT. | 500 mg per Pack Qty.
Art. No. 7528.2
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Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Activity: (Haemoglobin, pH 7.5; 25 °C) 30 mAnson-U/mg
Foreign activity: RNase and DNase not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5.
pH optimum: 8,0. Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Stock solution: 20 mg/ml in water
Storage temperature (stock solution): -20 °C
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
Working solution: 50 µg/ml
Proteinase K ≥30 mAnson U/mg, lyophilized
A non-specific protease with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
- Preparing chromosomal DNA for PFGE
- Degrading nucleases during preparation of nucleic acids (includes preparation of native RNA)
Art. No. 7528.3: A stock solution with a concentration of 20 mg/ml can be prepared directly by adding 25 ml buffer or water.
- Subtotal: 0.00
|Order No.||Pack Qty.||Pack.||Packaging||Price||Quantity|
|7528.1||100 mg||glass||1 x 100 mg||€93.45||
|7528.2||500 mg||glass||1 x 500 mg||€317.15||
|7528.3||500 mg||glass||for direct preparation of stock sol.||€353.70||
|7528.4||1 g||glass||1 x 1 g||€532.15||
|7528.5||250 mg||glass||1 x 250 mg||€177.40||
|7528.6||5 g||glass||1 x 5 g||€2,105.95||
Available at short notice
Delivery time on request
|Activity (Hemoglobin, 37 °C)||≥30 U/mg|
|DNases, RNases||not detected|