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3-Azido-7-hydroxycoumarin, 1 mg
From baseclick
≥98 %
All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
Pack Qty.
Pack.
3-Azido-7-hydroxy-chromen-2-one
Empirical formula C9H5N3O3
Molar mass (M) 203,15 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. [817638-68-9]
Molar mass (M) 203,15 g/mol
Storage temp. -20 °C
Transport temp. cooled
WGK 1
CAS No. [817638-68-9]
Fluorescent dye
For a 10 mM solution: add 492 µl to 1 mg.
For a 10 mM solution: add 492 µl to 1 mg.
€94.50
/Pack Qty.
€75.55/Pack Qty. Campaign price!
excl. VAT. | 1 mg per Pack Qty.
Art. No. 7811.1
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Product details
Technical Information
| Absorption max. | 404 nm (nach Click-Reaktion) |
| Emission max. | 480 nm (nach Click-Reaktion) |
| pKa (at 25 °C) | ≥98 % |
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3-Azido-7-hydroxycoumarin
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| Order No. | Pack Qty. | Pack. | Price | Quantity | ||
|---|---|---|---|---|---|---|
| 7811.1 | 1 mg | plastic | €75.55 |
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| 7811.2 | 5 mg | plastic | €253.25 |
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General information
Click Chemistry
The copper(I)‑catalysed azide-alkyne cycloaddition (CuAAC) is the most prominent example of a group of reactions named click reactions. According to Sharpless’ definition, these reactions are characterised by high yields, mild reaction conditions, and by their tolerance of a broad range of functional groups.[1–3] Typically, the reactions require simple or no workup or purification of the product. The most important characteristic of the CuAAC reaction is its unique bioorthogonality as neither azide nor terminal alkyne functional groups are generally present in natural systems.
References:
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
1 H. C. Kolb, M.G. Finn, K. B. Sharpless, Angew. Chem. Int. Ed. 2001, 40, 2004-2021.
2 C.W. Tornoe, C. Christensen, M. Meldal, J. Org. Chem. 2002, 67, 3057-3064.
3 V. V. Rostovtsev, L. G. Green, V. V. Fokin, K. B. Sharpless, Angew. Chem. 2002, 114, 2708-2711; Angew. Chem. Int. Ed. 2002, 41, 2596-2599.
Tags
Fluorescent Tags
Carl ROTH offers a selection of fluorescent tags for labelling of (bio-)molecules via click chemistry. All available tags are suitable for fluorescence spectroscopy applications due to their excellent absorption properties and good solubility. Tags with PEG-spacer are beneficial for applications with as little interaction between the analyte and the tag as possible. The coumarine derivative (Art No. 7811) offers a unique feature: fluorescence is only detected after the coupling via click reaction.
Analysis certificates
Type analysis
| Appearance | brown solid |
| Assay (HPLC) | ≥98 % |
| Absorption max. (buffer, pH 7) | 404 nm (after click reaction) |
| Emission max. (buffer, pH 7) | 480 nm (after click reaction) |
| Solubility | DMSO, DMF, MeOH, MeCN |