ROTIPHORESE®NF 10x TBE Buffer
Boiling point (bp) 100 °C
Storage temp. +15 to +25 °C
Transport temp. ambient temp.
Tested and optimised for automatic sequencers.
Concentrated TBE tends to precipitate salts during storage. Mix before use!
10x stock solu ...
excl. VAT. | 2.5 l per Pack Qty.
Art. No. A118.1
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ROTIPHORESE®NF 10x TBE Buffer ROTIPHORESE® 10x konz., fluorescence-free
10-times concentrated buffer formulated specially for automated sequencing.
10x stock solution contains 0,89 M Tris-Borate and 22 mM EDTA in distilled, deionised water; pH 8,3.
Suitable for manufacturing sequencing gels with ROTIPHORESE®NF Acrylamide/Bis-solution 40 % (19:1) and as running buffer.In sequencing, NF-TBE buffer is usually used in a final concentration of 1x.
TBE buffer has a higher buffer capacity than TAE, but also leads to stronger heating of the gels. A slightly lower voltage should therefore be selected when gelling. DNA migrates in the TBE gel about half as fast as in TAE-buffered gels.
ROTIPHORESE®TBE buffer has a high buffer capacity and is therefore ideally suited as a gel and running buffer system for sequencing gels.
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