Bromocresol green, 1 g
Molar mass (M) 698,04 g/mol
Density (D) 0,78 g/cm³
Melting point (mp) 218 °C
CAS No. [76-60-8]
excl. VAT. | 1 g per Pack Qty.
Art. No. T115.1
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|Colour conversion||yellow - blue|
|Transition range pH||3,8 – 5,4|
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On the following pages, you will find the determination of
• Nitrogen according to Kjeldahl
and the determination of the
• Iodine value
• Acid value
• Peroxide value
• Saponification value
as well as other determining factors such as
• Hydroxyl value
• Fluoride ions
The nitrogen is now present in the sulphuric acid as ammonium sulphate. Adding a strong base (such as NaOH) neutralises the sulphuric acid and liberates ammonia from the solution.NH4SO4 (solv) + 2 NaOH (aq) → Na2SO4 (aq) + 2 NH3 (g) + 2 H2O (l) The ammonia is led into an acid (such as boric acid) by means of steam distillation.B(OH)3 (aq) + 2 H2O (l) + NH3 (g) → B(OH)4– (aq) + NH4+ (aq) The resulting strong base (borate ion) is back-titrated with a strong acid (hydrochloric acid or sulphuric acid). The excess weak boric acid is not captured in the process. A Tashiro's indicator that changes colour in the acid is used for titration. The amount of acid that has been used up can then be converted into the amount of nitrogen in the sample. NH4+ (aq) + B(OH)4– (aq) + HCl (l) → NH4Cl (aq) + B(OH)3 (aq) + H2O (l)To calculate the protein content of the sample, the varying nitrogen content of the amino acids must be checked and the relevant conversion factors applied. The nitrogen contained in food derives mainly from proteins, but different samples may also contain other sources of nitrogen.
Thanks to the development of new sorbents and supports, as well as increasing instrumentation and automation, TLC has become a versatile separation method. It is used both in qualitative analysis and in quantitative analysis.
Applications range from simple manual separation processes in classic TLC to automated processes in HPTLC (high performance thin layer chromatography).
Advantages of thin layer chromatography:
• Higher sample throughput in less time
• Suitable for screening tests
• The ready-to-use TLC layer functions as a data storage device for separation results
• Pilot process for HPLC
• The separated substances can be used later for further analysis (e.g. IR, MS)
• By switching the mobile and the stationary phases, the separation process can be optimised quickly and cost-effectively
|Appearance||light tan powder|
|Appearance of solution||complies|
|pH-transition range||3,8 (yellow)-5,4 (blue)|
|Loss on drying (110 °C)||≤3 %|