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Amido black 10 B (C.I. 20470)

Pack Qty.
Naphthol blue black
Empirical formula C22H14N6Na2O9S2
Molar mass (M) 616,50 g/mol
CAS No. [1064-48-8]
EG-Nr. 213-903-1

For irreversible staining of proteins on membranes and for quantification of proteins in detergent containing solutions.

Compatible with PVDF and NC membranes.
Product details
Type analysis

€40.75/Pack Qty. 

excl. VAT. | 25 g per Pack Qty.

Art. No. 9590.1

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Product details

Amido black 10 B (C.I. 20470) p.a.

Compatible with PVDF and NC membranes.

Directions for use

Staining solution: 0.5 % amido black in 50 % methanol (Art. No. 4627.1) / 10 % acetic acid (Art. No. 3738.1). Stain for 1–5 min at room temperature. Reduce background in 50 % methanol at room temperature.

Technical Information
Ox. colour Yellow-brown  
Red. colour Blue  
Redox potential E0[V] at pH=0 and 20 °C +0.57  
Usage note for additional info Dissolve 0.2 g in 100 ml water  
Amido black 10 B (C.I. 20470)
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9590.1 25 g glass €40.75
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General information

Indicators and Dyes

Carl ROTH offers many indicators and dyes of high purity, which can be used in special applications.

Analysis certificates

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancedark-brown powder  
Dye content≥75 %  
Loss on drying≤10,0 %  

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