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ROTI®elution Tubes MAXI in stabilising solution, 1 unit(s)

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Examples of effect: Lead to damage to heath, cause irritation to eyes, skin or respiratory organs. Are fatal in larger quantities. Safety: As previously stated, in the event of skin irritation or contact with eyes, rinse with water or a suitable medium.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338 P337+P313
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water [or shower], IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing, if eye irritation persists: Get medical advice/attention
Related products
MWCO
Pack Qty.
Boiling point (bp) 78 °C
Melting point (mp) -114 °C
Storage temp. +4 °C
ADR 3 III
WGK 1
UN-Nr. 1170


RNase-free, DNase-free and proteinase-free, free of PCR products.

For gel elution and purification of DNA/RNA and proteins.

Product details

€20.35/Pack Qty. 

excl. VAT. | 1 unit(s) per Pack Qty.

Art. No. 9489.1

In stock
Free of shipping costs from 125 €
24 hour direct shipment
from 6 Pack Qty. €19.33/Pack Qty.
from 24 Pack Qty. €18.32/Pack Qty.
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Product details


RNase-free, DNase-free and proteinase-free, free of PCR products.

For gel elution and purification of DNA/RNA and proteins.

  • Ultrapure
  • Easy-to-handle
  • Very gentle process
  • Typical recovery rate of >95%
  • Suitable for elution from agarose and PAGE
  • Suitable for dialysis of biomolecules
  • Suitable for oligoncleotides, dsDNA, ssDNA, RNA, proteins and protein/nucleic acid complexes
  • Compatibel with all popular electrophoresis units

Easy-to-handle system for gel elution and purification of DNA/RNA and proteins in solution (electro elution).

This very gentle method guarantees the non-disruptive purification of high-molecular or genomic DNA as well as of native protein complexes and long peptides. Using membranes of low MWCO, elution of very small DNA fragments is possible, making the tubes a very useful tool for the efficient desalting of primers within 60-100 min as well as for purification of siRNA (ss or duplexes).

ROTI®elution tubes are suitable for elution of ssDNA (≥20 nt), dsDNA (15 bp-100 kb), RNA of each size, proteins of each size, and of protein/nucleic acid complexes. The tubes may be applied to elution procedures from agarose- and polyacrylamide gels, as well as to all popular electrophoresis units. Used in floating racks, they are ideal for the purification of DNA, RNA or proteins by means of dialysis.

All ROTI®elutions tubes are free of DNase, RNase, Proteinase and PCR products. All membranes are made from regenerated cellulose and can thus be exposed to a pH range of 2-12, to a temperature of +60 °C, and also to a variety of organic solvents. Additionally, the membranes are free of sulphate and heavy metals and were treated with EDTA. Hence, macromolecules (like proteins or nucleic acids) or other biological particles may be eluted and dialysed in native conformation, and are very well suited for subsequent activity tests.


Directions for use

ROTI®elution tubes are very easy to handle, and therefore can be used in both individual tests and high-throughput applications. Thanks to the screw cap, the vials are easy to open, can easily be filled with gel slices and buffer and can be securely sealed.

The practical elution vial holder has sufficient stability for safe use in electrophoresis cell. Under current flow, the DNA/RNA/protein molecules initially migrate from the gel slice until they are halted by the dialysis membrane. By briefly reversing the polarity of the electrical field, the molecules can be separated from the membrane and pipetted out of the tube together with the buffer. By carefully selecting the tube and the amount of buffer, it is in many cases possible to achieve a high concentration directly within the elution buffer. The buffer can then easily be concentrated by using the usual methods (e.g. precipitation).

ROTI®elution tubes with MWCO 1, 25 and 50 kDa are wet packed in stabilising solution (25 % ethanol, 2 mM EDTA) and should stored under refrigeration. All other elution vials are dry packed and can be stored at room temperature.


The classification below refers only to Art. Nos. marked *.

ROTI®elution Tubes MAXI in stabilising solution 

Technical Information
MWCO 50 kDa
Delivery form In ethanol solution 
Additional info Recommended storage temperature for dry elution tubes: +15-25 °C; for elution tubes in solution: +4 °C 
Type MAXI 50* 
ROTI®elution Tubes MAXI in stabilising solution
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Order No. MWCO Pack Qty. Price Quantity
9489.2 50 kDa 5 unit(s) €94.50
9489.3 50 kDa 30 unit(s) €392.40
9489.1 50 kDa 1 unit(s) €20.35
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