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Nile blue A (C.I. 51180), 10 g

From  Carl ROTH
~80 %
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Nile blue sulphate
Empirical formula C40H40N6O6S
Molar mass (M) 732,84 g/mol
Melting point (mp) >300 °C
WGK 1
CAS No. 3625-57-8
EG-Nr. 222-832-5

Dissolve 0.1 g in 100 ml of water
Redox indicator

Lipid staining dye, soluble in water (acidulated with H2SO4). It stains neutral lipids pink and acid lipids blue.
Product details

€38.60/Pack Qty. 

excl. VAT. | 10 g per Pack Qty.

Art. No. 4377.1

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Product details



Nile blue A (C.I. 51180) ~80 %

Lipid staining dye, soluble in water (acidulated with H2SO4). It stains neutral lipids pink and acid lipids blue.



Technical Information
Application saturated aqueous solution (acidulated with H2SO4
Dyeing dead cells blue 
Absorption maximum 633 nm (in 50% EtOH) 
Colour conversion blue - violet-red 
Soluble in Fat/oil 
Detection neutral lipids pink, acid lipids blue 
Ox. colour blue-red 
Transition range pH 10,2 - 13,0 
Red. colour colourless 
Redox potential E0[V] at pH=0 and 20 °C +0.41 
Use vitality test 
Nile blue A (C.I. 51180)
Selected quantity:   0
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Art. No. Pack Qty. Pack. Price Quantity
4377.1 10 g glass

€38.60

4377.2 5 g glass

€24.20

4377.3 25 g glass

€75.80

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  1. Subtotal:  0.00

Downloads / MSDS


General information

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The content of colourants is not specified for many products for various reasons:

1. complex composition: colourants are often mixtures of several components that may be present in different ratios. These compositions can vary, which makes it difficult to state an exact content.

2. application focus: In many applications, especially in the textile and food industries, the exact concentration of a dye is less relevant than its colouring power or its ability to achieve a certain colour. Here, the colour strength or the ability to impart colour is the most important criterion.

3. standardisation: Many dyes are traded according to certain standards (e.g. C.I. numbers) which guarantee a certain quality and colouring. These standards often make an exact specification of content superfluous, as users know that the colourant has the desired properties.

4. manufacturing processes: The manufacturing process of colourants can lead to natural variations in content. Instead of specifying an exact content, quality control is often carried out to ensure that the colourant is within an acceptable range.

5. Regulatory aspects: In some cases, regulatory requirements may not require the manufacturer to declare the exact content as long as the colourant is safe and effective for the intended purpose.

6. cost reasons: An exact determination of the content often requires complex analytical procedures that could increase production costs. Therefore, some manufacturers do not provide this additional information in order to save costs.

In summary, it can be said that the content of colourants is often not specified because other quality criteria are in the foreground and the exact concentration is not decisive for many applications.


Certificates of Analysis

You can search for and download your certificate of analysis for the selected product here. Please provide your batch number.
The following analysis certificates have been found:

Type analysis

Appearancedark green to black powder
Dye content~80 %
Suitability for microscopycomplies