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ROTI®Load DNA 1x (with glycerol)

1x conc., ready-to-use, DNase-free
Icon_ready-to-use Ready-to-use products and reagents
Icon_DNAse-free
Verwante producten
VE
Verp.
Verpakking
Density (D) 1 g/cm³
Boiling point (bp) 100 °C
Storage temp. -20 °C
Transport temp. ambient temp.
WGK 1

DNA-gel loading buffer for DNA gel electrophoresis of routine gels, excellent for resolubilisation of DNA pellets.
Contains: Tris, Na-acetate, EDTA, bromophenol blue, xylene cyanol and glycerol

The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.
Productdetails

47,85 €/VE 

Excl. btw | 5 ml Per VE

Bestelnr. 0100.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!
Vanaf 6 VE 45,46 €/VE
Vanaf 24 VE 43,06 €/VE

Productdetails


The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.


ROTI®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.


Arbeidsconcentratie

ROTI®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI®Load DNA. ROTI®Load DNA gel loading buffer is usually used as 1x concentrated solution.



ROTI®Load DNA 1x (with glycerol) 1x conc., ready-to-use, DNase-free
Gebruiksinstructies

In order to cover the main size reange needed in gel electrophoresis, ROTI®Load DNA 1x (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image).


Glycerin is the standard reagent for increasing sample density.


Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.



Technische informatie
Kleurstof Bromphenol blue, Xylene cyanole 
Incl. DNA-kleuring nee 
Toepassing Standard applications, pelleted DNA 
ROTI®Load DNA 1x (with glycerol)
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
0100.1 5 ml plastic 5 x 1 ml

47,85 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

Selecting a Suitable ROTI®Load Gel Loading Buffer for Nucleic Acids

Care should be taken to ensure that the dyes contained in the gel stop before smallest relevant DNA bands. This ensures that the buffer can be stopped in time. However, the selected dyes may overlay the bands shown. In this case, select a loading buffer that does not contain any unwanted dyes.
Bromophenol blue and xylene cyanol can be used as colour markers in all standard gels. If a relevant band is overlaid by one of these colour markers, choose a gel loading buffer containing Orange G. If small fragments are to be assayed, the gel loading buffer should contain Orange G in order to mark the run. If relevant bands are overlaid, however, a choice can be made between bromophenol blue or xylene cyanol depending on the size of the bands. A loading buffer without xylene cyanol is usually used for assaying large fragments.

Glycerine is the standard reagent for increasing the density of samples.
Ficoll® 400 produces particularly well-defined bands.


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Notes on the product

Each batch is functionally tested for its suitability in electrophoresis and for DNAse-freeness.