ROTI^®Prep Gel Extraction, 250 preparations

DNA extraction from agarose gels through incubation and subsequent spin column process.
The gel slice to be extracted (up to 300 mg) is first solubilised in a lysis buffer through a simple 10 minute incubation. After that, DNA is elutied via convenient spin filter centrifugation in approx. 10 mins.
A specially developed Binding Enhancer is admixed in order to enhance binding efficiency of the DNA to the filter, and thus yield and purity of the isolated DNA.

Furthermore, the ROTI^®Prep Gel Extraction kit allows, if needed, to reduce elution volume down to 10 μl in order to obtain particularly highly concentrated DNA.
For gel runs both, TAE and TBE may be used. The kit is compatible with all standard gel loading buffers and gel staining reagents, even non-toxic ethidium bromide replacements like, for instance, Roti^®GelStain.
Isolated DNA is free of residual agarose, PCR- and ligase inhibitors, and cytotoxic reagents, and can directly be applied to all standard downstream applications like cloning, PCR, transformation and transfection.

Binding Enhancer, Gel Solubizer, Washing Solution WSL, Elution Buffer EB, spin columns, collection tubes, elution tubes, detailed instructions-for-use.
Contents of this Kit may not be bought separately.

Please order ethanol 96-99,8 % (p.a., Art. No. 9065) separately.