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ROTI®Garose-His/Co Beads, 25 ml

ROTI®Garose for biochemistry
Icon_EVE-EUD Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Mogelijke gevolgen: zijn ontvlambaar; vloeistoffen vormen met lucht explosieve mengsels; zorgen met water voor ontvlambare gassen of zijn zelfontvlambaar. Veiligheid: uit de buurt van open vuur en warmtebronnen houden; vaten dicht afsluiten; brandveilig bewaren.
Mogelijke gevolgen: leiden tot schade aan de gezondheid, irriteren organen, huid of luchtwegen. Leiden in grotere hoeveelheden tot de dood. Veiligheid: zoals hierboven; bij huidirritaties of oogcontact met water of geschikt middel spoelen.
Mogelijke gevolgen: veroorzaken allergieën, kankerverwekkend (carcinogeen), verandering erfelijk materiaal (mutageen), brengt de voortplanting in gevaar en vruchtbeschadigend (reprotoxisch) of orgaanbeschadigend Veiligheid: voor het werk met dergelijke stoffen moet men zich goed informeren; veiligheidskleding en handschoenen, oog- en mondbescherming of adembescherming dragen.
Mogelijke gevolgen: zijn voor waterorganismen schadelijk, giftig of zeer giftig, acuut of met langdurige werking. Veiligheid: alleen met gevaarlijk afval afvoeren, in geen geval in het milieu laten terechtkomen.
Danger
H226-H319-H350-H411-EUH208
i flammable liquid and vapour, causes serious eye irritation, may cause cancer, toxic to aquatic life with long lasting effects
P202 P210 P273 P280 P303+P361+P353 P308+P313
i do not handle until all safety precautions have been read and understood, keep away from heat. No smoking, avoid release to the environment, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF exposed or concerned: Get medical advice/attention
VE
Verp.
Cobalt agarose beads, Agarose beads for affinity chromatography, IMAC matrix/cobalt charged, Column matrix for protein isolation, IMAC beads
Density (D) ~0,7 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by affinity chromatography.
For batch mode and gravity flow

For the isolation of high yields of highly pure His-tagged proteins under non-reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Co Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.
Productdetails

385,95 €/VE 

Excl. btw | 25 ml Per VE

Bestelnr. 1235.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 3-4 weken
Vanaf 6 VE 366,65 €/VE
Vanaf 24 VE 347,36 €/VE

Productdetails


For the isolation of high yields of highly pure His-tagged proteins under non-reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Co Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified. The ready-to-use columns and cartridges offer you the perfect solution for fast and easy purification of His-tag proteins from total lysate.

  • Good recovery rate of extremely pure His-tagged proteins
  • Very low Cobalt leaching
  • Compatible with denaturing reagents

The matrix of ROTI®Garose His/Co products consists of crosslinked and beaded 6 % agarose, IDA-conjugated and charged with divalent Cobalt ions. Cobalt chelates recognize two histidines consecutive in an amino acid sequence with superior specificity and good affinity, resulting in acceptable to good recovery rates only, but yielding proteins of superior purity. This matrix is, therefore, the perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated. ROTI®Garose His/Co beads result in eluates with considerably low metal contamination.


Gebruiksinstructies

The tridentate IDA cross-linker provides easy elution with low amounts of imidazole. Additionally, ROTI®Garose Beads may repeatedly be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride, 5 mM DTT).
For insulation under reducing conditions we recommend ROTI®Garose-His/Ni NTA beads.



ROTI®Garose-His/Co Beads ROTI®Garose for biochemistry

Cobalt charged IDA-agarose beads for low pressure affinity chromatography.
50 % bead suspension in 20 % ethanol.

  • One-Step purification of His-tagged proteins from total lysates
  • Binding capacity (purified 6xHis protein) of 135 mg per ml matrix
  • Easy elution and regeneration
  • For batch mode and gravity flow column chromatogrphy

Geschikt voor

Perfect choice if extremely pure proteins are required, or if hard-to-separate proteins shall be isolated.


Gebruiksinstructies

May be autoclaved at 121 °C for 30 mins.



Technische informatie
Gebruikstip Bead suspension, binding capacity 135 mg/ml packed matrix (His)6 
ROTI®Garose-His/Co Beads
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1235.1 25 ml plastic

385,95 €

1235.2 100 ml plastic

1.048,15 €

Op voorraad
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In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)6 %
LigandIDA
Metal ionCobalt
Binding capacity (DHAK-6xHis)135 mg/ml
Stabilizer20 % ethanol