0180 - 51 67 04 (Nederland)

Roti®garose-Protein A Beads

for biochemistry
For purification of immunoglobulins by affinity chromatography.
Agarose beads for affinity chromatography, IMAC matrix/Protein A conjugated, Column matrix for protein isolation

Roti<sup>®</sup>garose-Protein A Beads
Storage temp.: +4 °C
ADR 3 III • WGK 1
UN-Nr. 1170

Protein A coated agarose beads for low pressure affinity chromatography. High-performance affinity resin for antibody purification.

  • One-Step purification of immunoglobulins from cell lysates and biological solutions
  • Binding capacity (human IgG) of approx. 25 mg per ml matrix
  • Superior affinity for IgG from various mammalian species
  • High yield of very pure immunoglobulins
  • Covalent binding of protein A in high ligand density
  • For batch mode and gravity flow column chromatography

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. Roti®garose Protein A Beads offer the perfect solution for isolation of your specific antibodies, either in low flow column chromatography or in batch mode.

The matrix of Roti®garose Protein A Beads consists of cross-linked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus. The recombinant protein A has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM. recProtein A shares IgG binding properties with natural protein A of S. aureus Cowan strain I. Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3.0).

Slurry in 20 % ethanol.


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Warning H226-H319

Roti®garose-Protein A Beads

Status Bestellingsnr. Verp Verpakking Prijs Hoeveelheid Actie
Artikel uit voorraad leverbaar 1278.1 5 ml plastic € 590,20
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Artikel binnenkort leverbaar 1278.2 25 ml plastic € 1.998,45
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Technische informatie

Type analysis:
Particle size50-150 µm
Beads (cross-linked agarose)4 %
Ligandrecombinant protein A
Chemical bondingcovalent
Ligand density (Protein A)ca. 3 mg/ml
Binding capacity (humane IgG)ca. 25 mg/ml
Stabilizer20 % ethanol

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