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ROTIPHORESE®Blue R
Ready-to-use products and reagents
Please consider that this product might be liable to some legal sanctions as for example to obtain an end user declaration or the prohibition of some applications. For details please do contact your counterpart in our sales department!
Boiling point (bp) >65 °C
Flash point (flp) >10 °C
ADR 3 II
WGK 2
UN-Nr. 3286
600 ml concentrated solution is sufficient for approx. 12 mini gels.
ROTIPHORESE®Blue R is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture.
40,35 €/VE
Excl. btw | 600 ml Per VE
Bestelnr. 3074.1
Geschikt voor / Toebehoren
Productdetails
ROTIPHORESE®Blue R ROTIPHORESE® concentrate
Concentrate for easy preparation of Coomassie® Blue R staining solutions.
ROTIPHORESE®Blue R is ideal for staining proteins in SDS-PAGE gels. It saves any tedious weighing of staining powder and having to prepare the mixture.
Gel stained by ROTIPHORESE®Blue R, 12% PAA in Tris, ROTI®Mark STANDARD (Art. No. T851).
Concentrate contains Coomassie® Blue R, methanol and pure acetic acid. ROTIPHORESE®Blue R can be diluted directly in the bottle with 400 to 600 ml deionized water, resulting in the following composition depending on the dilution.
When diluted with 397,5 ml water (to a total of 1 litre staining solution): Methanol (50 %), acetic acid 10 %, Coomassie® R (0,25 %)
When diluted with 507,5 ml water (to a total of 1,1 litre staining solution): Methanol (45 %), acetic acid 9 %, Coomassie® R (0,22 %)
When diluted with 602,5 ml water (to a total of 1,2 litre staining solution): Methanol (41,5 %), acetic acid 8,3 %, Coomassie® R (0,2 %).
We recommend mixing the concentrated solution well before dilution and filtering the diluted solution before application.
| Sensitiviteit | 50 |
| Toepassing | Coomassie® staining solution concentrate for rapid staining |
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 3074.1 | 600 ml | glass |
40,35 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
| Product | Sensitivity | Rapid Gel Analysis |
Gel Drying |
Elution/ Transfer |
2D- Gels |
MS- Analysis |
| ROTI®Black P | 1 ng/mm2 | - | +++ | - | +++ | - |
| ROTI®Blue | 10 ng/mm2 | - | +++ | +++ | - | +++ |
| ROTI®Blue quick | 10 ng/mm2 | +++ | +++ | ++ | - | ++ |
| ROTI®Blau R | 50 ng/mm2 | +++ | + | - | - | - |
| +++: recommended +: suitable -: not recommended | ||||||
Summary Staining of Protein Gels
| Product | Sensitivity | Description |
| ROTI®Black P | 1 ng/mm2 | Silver staining kit. Highly sensitive. Simple, fast and reproducible. |
| ROTI®Blue | 10 ng/mm2 | Coomassie®-solution for colloidal staining of proteins. Destaining not required. Non-fixing staining for protein gel elution possible. Proteomics-compatible. |
| ROTI®Blue quick | 10 ng/mm2 | Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. |
| ROTIPHORESE®Blau R | 50 ng/mm2 | Coomassie® staining solution concentrate for rapid staining. |
| Coomassie® Blue G250 (C.I. 42655) | 60 ng/mm2 | Dye for performing Coomassie® staining. ODmax = 595 |
| Coomassie® Blue R250 (C.I. 42660) | 50 ng/mm2 | Dye for performing Coomassie staining. ODmax = 550 |