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ROTITEST®Vital, 5 ml, 1 x 5 ml

ROTITEST® BioAnalysis Grade, sterile, ready-to-use
Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Steriel
VE
Verp.
Verpakking
Cell proliferation test solution
Density (D) 1,37 g/cm³
Boiling point (bp) 100 °C
Storage temp. +4 °C
WGK 1

Colorimetric test solution for evaluation of cell proliferation and -viability. Non toxic.
Stable at ambient temperature. Very stabile when being cooled.
Store protected from light. Do not freeze.

1 ml is sufficient for 100 measurements.

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.
Productdetails

224,70 €/VE 

Excl. btw | 5 ml Per VE

Bestelnr. 0069.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!
Vanaf 6 VE 213,46 €/VE
Vanaf 24 VE 202,23 €/VE

Productdetails

Steriel

Rapid and simple test system for non-radioactive quantitation of proliferating cells. Also well suitable for cytotoxicity assays.

Optimale vervanging voor MTT:
  • Cells keep on being vital
  • Most simple handling
  • One-component-system without radioactivity
  • For adherent and suspension cells
  • Photometric result in 1 to 4 hours
  • Strong correlation between absorbance and cell number
  • Suitable for every culture media


ROTITEST®Vital ROTITEST® BioAnalysis Grade, sterile, ready-to-use
Mechanisme

Sensitivity of the tetrazolium salt used is higher than that known for standard reagents such as MTT, XTT, MTS, or WST-1. Results were shown to be of highly stringent correlation with [3H]-thymidine incorporation assays, therefore representing viability as well as proliferation status of the cells (Tominaga et al., Anal. Commun. 1999 (36) 47-50).
In all living cells, NADH and NADPH are formed by the respiratory chain. ROTITEST®vital is based on the (colourless) WST-8, which functions as acceptor for the NADH/NADPH dehydrogenase while being reduced to (coloured) formazane during the process.

0069_RotitestVital-Ablaufschema
0069_RotitestVital-Substanztest-Ablaufschema

Gebruiksinstructies

The test solution is added directly to the cultivated cells in 96wells. During incubation for few hours, a water-soluble formazan dye is produced by viable cells, and is released into the surrounding medium. The resulting orange colour is measured photometrically (approx. 450 nm).
Also suitable for culture media containing phenol red.

Each package contains simple-to-follow instructions-for-use.

0069_Anwendung
Fig.: Example of use

0069_Diagramm_A
Fig. A: Correlation of cell counting results achieved with ROTITEST® Vital (10 μl/well) and 3H-Thymidine (37 KBq/well). HeLa (1, in DMEM) and HL60 cells (2, in MEM).
0069_Diagramm_B
Fig. B: Comparison of cell counting results using ROTITEST® Vital (10 μl/well) and other cell counting reagents. Photometrical measurement at 450 nm (ROTITEST® Vital, XTT), 490 nm (MTS), and 570 nm (MTT). HeLa (1, in DMEM) and HL60 cells (2, in MEM).
0069_Diagramm_CD
Fig. C: Measurement of cytotoxic effects of actinomycin D (exposition 24 h) on HeLa cells (in DMEM), assayed with ROTITEST® Vital (10 μl/well).

Fig. D: Proliferation assay on CTLL-2 cells (in RPMI) under influence of humane IL-2 (exposition 72 h), using ROTITEST® Vital (10 μl/well).


ROTITEST®Vital
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
0069.1 5 ml plastic 1 x 5 ml

224,70 €

0069.2 20 ml plastic 4 x 5 ml

671,90 €

0069.3 1 ml plastic 1 x 1 ml

80,55 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Appearance (test solution)red
Turbiditytransparent
Absorptioncomplies