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Proteinase K, 100 mg, 1 x 100 mg
WGK 1
CAS No. 39450-01-6
EG-Nr. 254-457-8
Stock solution: 20 mg/ml in water. Storage temperature (stock solution): -20 °C.
Art. No. 7528.3: A The stock solution (20 mg/ml) can be prepared directly in the bottle.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
96,20 €/VE Campaign price!
Excl. btw | 100 mg Per VE
Bestelnr. 7528.1
Productdetails
Proteinase K (from Tritirachium album) is a non-specific protease of the serine protease family.
Proteinase K is used for the cleavage of proteins in nucleic acid preparations. It is mainly used in nucleic acid purification or for the removal of nucleases.
Proteinase K is active under a wide range of reaction conditions, including elevated temperatures and presence of SDS.
Foreign activity: RNAse and DNAse not detectable
Optimum temperature: +65 °C.
Activity at +65 °C is ca. 12 x higher than at +25 °C. Over +65 °C, inactivation due to denaturation.
Activators: Denaturating agents like SDS (0,5-1 %), urea.
Inhibitors: Inhibition with Hg2+-ions, DFP, PMSF and phenol. Not inhibited by EDTA, sulfhydryl reagents and trypsin or chymotryps ininhibitors.
Stability: pH 4.0-12.5. pH optimum: 8,0.
Also stable even when denaturing agents, e.g. SDS and urea are present.
Stabilisers: Ca2+-ions (1-5 mM) prevent autolysis.
Proteinase K ≥30 mAnson U/mg, BioAnalysis Grade, lyophilized
A non-specific endopeptidase with strong proteolytic activity for degrading proteins in biological samples. A quality product for molecular biology with a broad scope of application.
Working solution: 50 µg/ml
Reaction buffer: 50 mM Tris-HCl; pH 7.5; 5 mM CaCl2; 0.5 % SDS
- Tussentotaal: 0.00
Bestelnr. | VE | Verp. | Verpakking | Prijs | Hoeveelheid | |
---|---|---|---|---|---|---|
7528.1 | 100 mg | glass | 1 x 100 mg |
120,30 €
96,20 € |
|
|
7528.2 | 500 mg | glass | 1 x 500 mg |
407,45 €
325,90 € |
|
|
7528.3 | 500 mg | glass | in 30 ml glass vial |
446,15 €
356,90 € |
|
|
7528.4 | 1 g | glass | 1 x 1 g |
671,90 €
537,50 € |
|
|
7528.5 | 250 mg | glass | 1 x 250 mg |
224,70 €
179,70 € |
|
|
7528.6 | 5 g | glass | 1 x 5 g |
2.682,15 €
2.145,70 € |
|
|
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Leveringsdatum onbekend
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)
There are six classes in which all enzymes are classified according to the particular reaction they catalyse:
• Oxidoreductases (catalyse redox reactions)
• Transferases (transfer functional groups among substrates)
• Hydrolases (cleave bonds via addition of water)
• Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)
• Isomerases (transform chemical isomers)
• Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)
Analysecertificaten
Guarantee analysis
Activity (Hemoglobin, 37 °C) | ≥30 U/mg |
DNases, RNases | not detected |
Endonucleases | not detected |
Assay DNA | ≤10 pg/mg |
Solubility (c=20 mg/ml in H2O) | clear, colorless |