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Elution Tubes ROTI® MIDI, 3,5 kDa, 2 stuks

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MWCO
VE
Volumebereik tot
Storage temp. +15 to +25 °C


For gel elution and purification of DNA/RNA and proteins.

Easy-to-handle system for gel elution and purification of DNA/RNA and proteins in solution (electro elution).

This very gentle method guarantees the non-disruptive purification of high-molecular or genomic DNA as well as of native protein complexes and long peptides. Using membranes of low MWCO, elution of very small DNA fragments is possible, making the tubes a very useful tool for the efficient desalting of primers within 60-100 min as well as for purification of siRNA (ss or duplexes).

ROTI®elution tubes are suitable for elution of ssDNA (≥20 nt), dsDNA (15 bp-100 kb), RNA of each size, proteins of each size, and of protein/nucleic acid complexes. The tubes may be applied to elution procedures from agarose- and polyacrylamide gels, as well as to all popular electrophoresis units. Used in floating racks, they are ideal for the purification of DNA, RNA or proteins by means of dialysis.

All ROTI®elutions tubes are free of DNase, RNase, Proteinase and PCR products. All membranes are made from regenerated cellulose and can thus be exposed to a pH range of 2-12, to a temperature of +60 °C, and also to a variety of organic solvents. Additionally, the membranes are free of sulphate and heavy metals and were treated with EDTA. Hence, macromolecules (like proteins or nucleic acids) or other biological particles may be eluted and dialysed in native conformation, and are very well suited for subsequent activity tests.
Productdetails

20,35 €/VE 

Excl. btw | 2 stuks Per VE

Bestelnr. 9266.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!
Vanaf 6 VE 19,33 €/VE
Vanaf 24 VE 18,32 €/VE

Productdetails


For gel elution and purification of DNA/RNA and proteins.

Easy-to-handle system for gel elution and purification of DNA/RNA and proteins in solution (electro elution).

This very gentle method guarantees the non-disruptive purification of high-molecular or genomic DNA as well as of native protein complexes and long peptides. Using membranes of low MWCO, elution of very small DNA fragments is possible, making the tubes a very useful tool for the efficient desalting of primers within 60-100 min as well as for purification of siRNA (ss or duplexes).

ROTI®elution tubes are suitable for elution of ssDNA (≥20 nt), dsDNA (15 bp-100 kb), RNA of each size, proteins of each size, and of protein/nucleic acid complexes. The tubes may be applied to elution procedures from agarose- and polyacrylamide gels, as well as to all popular electrophoresis units. Used in floating racks, they are ideal for the purification of DNA, RNA or proteins by means of dialysis.

All ROTI®elutions tubes are free of DNase, RNase, Proteinase and PCR products. All membranes are made from regenerated cellulose and can thus be exposed to a pH range of 2-12, to a temperature of +60 °C, and also to a variety of organic solvents. Additionally, the membranes are free of sulphate and heavy metals and were treated with EDTA. Hence, macromolecules (like proteins or nucleic acids) or other biological particles may be eluted and dialysed in native conformation, and are very well suited for subsequent activity tests.

  • Ultrapure
  • Easy-to-handle
  • Very gentle process
  • Typical recovery rate of >95%
  • Suitable for elution from agarose and PAGE
  • Suitable for dialysis of biomolecules
  • Suitable for oligoncleotides, dsDNA, ssDNA, RNA, proteins and protein/nucleic acid complexes
  • Compatibel with all popular electrophoresis units

Toepassingsvoorbeelden

Purification of DNA and proteins from gels, elution of genomic DNA or total RNA, preparation of DNA fragments prior to cloning, purification of siRNA for RNAi assays, purification/desalting of oligonucleotides/primers, purification of RNA or DNA for transfection or transformation, desalting or buffer change of protein solutions.

Please order the respective ROTI®elution Tube Tray separately.


Gebruiksinstructies

ROTI®elution tubes are very easy to handle, and therefore can be used in both individual tests and high-throughput applications. Thanks to the screw cap, the vials are easy to open, can easily be filled with gel slices and buffer and can be securely sealed.

The practical elution vial holder has sufficient stability for safe use in electrophoresis cell. Under current flow, the DNA/RNA/protein molecules initially migrate from the gel slice until they are halted by the dialysis membrane. By briefly reversing the polarity of the electrical field, the molecules can be separated from the membrane and pipetted out of the tube together with the buffer. By carefully selecting the tube and the amount of buffer, it is in many cases possible to achieve a high concentration directly within the elution buffer. The buffer can then easily be concentrated by using the usual methods (e.g. precipitation).

ROTI®elution tubes with MWCO 1, 25 and 50 kDa are wet packed in stabilising solution (25 % ethanol, 2 mM EDTA) and should stored under refrigeration. All other elution vials are dry packed and can be stored at room temperature.


The classification below refers only to Art. Nos. marked *.

Elution Tubes ROTI® MIDI 

Technische informatie
Type MIDI 3.5 
Inhoud 50 tot 800 µl
MWCO 3,5 kDa
Gesch. voor Gel pieces up to approx. 0.5 x 1.2 cm 
Levervorm droog 
Aanvullende informatie Recommended storage temperature for dry elution tubes: +15-25 °C; for elution tubes in solution: +4 °C 
Elution Tubes ROTI® MIDI
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. MWCO VE Volumebereik tot Prijs Hoeveelheid
9266.1 3,5 kDa 2 stuks 800 µl

20,35 €

9266.2 3,5 kDa 30 stuks 800 µl

203,20 €

9266.3 3,5 kDa 100 stuks 800 µl

590,20 €

9294.1 8 kDa 2 stuks 800 µl

20,35 €

9294.2 8 kDa 30 stuks 800 µl

198,90 €

9294.3 8 kDa 100 stuks 800 µl

590,20 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Algemene informatie

Recommendation for the Choice of the MWCO:

MWCO (kDa) RNA/ssDNA dsDNA Proteins
1 20-50 nt 15-25 bp 3-15 kDa
3,5 50-250 nt 25-100 bp 10-30 kDa
8 250-1000 nt 100-500 bp 25-50 kDa
14 1-5 knt 0,5-2 kb 45-100 kDa
25 5-30 knt 2-10 kb 80-200 kDa
50 >30 knt 10-100 kb >150 kDa