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ROTI®Garose-Glu/GST Cartridges, 5 ml, 1 x 5 ml

ROTI®Garose for biochemistry
Icon_ready-to-use Ready-to-use products and reagents
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Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
VE
Verp.
Verpakking
Glutathione-agarose beads, Affinity chromatography colums, Column matrix for protein isolation
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of GST-tagged proteins by high pressure affinity chromatography
Suitable for MPLC, FPLC, ÄKTA™ and peristaltic pumps

Glutathione coupled agarose beads for isolation of glutathione-S-transferase (GST) or GST fusion proteins.

ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
Productdetails

532,15 €/VE 

Excl. btw | 5 ml Per VE

Bestelnr. 0843.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 3-4 weken
Vanaf 6 VE 505,54 €/VE
Vanaf 24 VE 478,94 €/VE

Productdetails


Glutathione coupled agarose beads for isolation of glutathione-S-transferase (GST) or GST fusion proteins.

ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • Rapid, mild and highly specific purification
  • Suitable for isolation of GST-peptides and protein complexes of all sizes
  • High binding capacity
  • Easy elution and regeneration

Efficient and highly specific isolation of GST-tagged proteins via affinity chromatography is based on the affinity of the glutathion-S-transferase to it’s substrate glutathione. Merely no unspecific binding of proteins without GST-tag takes place, even during purification from raw extracts, while the biological activity of the purified proteins is preserved by chromatography conditions.


Gebruiksinstructies

The matrix of ROTI®Garose-Glu/GST Beads consists of beaded 4 % agarose without crosslinking, coupled with glutathione. Elution is carried out via a mild Tris/glutathione buffer, enabling even recovery of native protein complexes. ROTI®Garose-Glu/GST Beads may be regenerated, making them very cost-effective.



ROTI®Garose-Glu/GST Cartridges ROTI®Garose for biochemistry

Prepacked cartridges with glutathione coupled agarose beads for medium pressure affinity chromatography (MPLC).
ROTI®Garose-Glu/GST Cartridges offer the perfect solution for your specific application of chromatography under medium pressure (MPLC, FPLC).
Matrix in 20 % ethanol.

  • Easy isolation of GST-tagged proteins from total lysates
  • Recommended flow rate 0,5-5 ml/min.
  • Standard ports (10-32)
  • For medium pressure flow chromatography
  • Very versatile. Applicable with ÄKTA™FPLC™, pump and syringe

Geschikt voor

Optimal for isolation of glutathione-S-transferase (GST) or GST fusion proteins via automated liquid chromatography, or if proteins shall be isolated under pressure. Suitable for ÄKTA™FPLC™ and for sample application via peristaltic pump or syringe.


Gebruiksinstructies

ROTI®Garose-Glu/GST Cartridges are prepacked and may be used directly.

Column material made from polypropylene and polyethylene (frit), Inner diameter 1,6 cm, height 2,5 cm, inlet port 10-32 (1/16″) female, outlet port 10-32 (1/16″) male.



Technische informatie
Gebruikstip Prepacked columns, binding capacity ≥50 mgl/column 
ROTI®Garose-Glu/GST Cartridges
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
0843.1 5 ml plastic 1 x 5 ml

532,15 €

0843.2 25 ml plastic 5 x 5 ml

2.144,65 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Column volume5 ml
Particle size50-150
Beads (un-cross-linked agarose)4 %
LigandGlutathione
Binding capacity/cartridge (GSTrek)≥50 mg