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ROTI®Garose-His/Ni NTA-Beads, 25 ml

ROTI®Garose for biochemistry
Mogelijke gevolgen: zijn ontvlambaar; vloeistoffen vormen met lucht explosieve mengsels; zorgen met water voor ontvlambare gassen of zijn zelfontvlambaar. Veiligheid: uit de buurt van open vuur en warmtebronnen houden; vaten dicht afsluiten; brandveilig bewaren.
Mogelijke gevolgen: leiden tot schade aan de gezondheid, irriteren organen, huid of luchtwegen. Leiden in grotere hoeveelheden tot de dood. Veiligheid: zoals hierboven; bij huidirritaties of oogcontact met water of geschikt middel spoelen.
Warning
H226-H319-EUH208
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P303+P361+P353 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF ON SKIN (or hair): Take off immediately all contaminated clothing. Rinse skin with water, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
VE
Verp.
Nickel agarose beads, Agarose beads for affinity chromatography, IMAC matrix/nickel charged, Column matrix for protein isolation, IMAC beads
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For isolation of His-tagged proteins by affinity chromatography under reducing conditions
For batch mode and gravity flow

For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
Productdetails

471,95 €/VE 

Excl. btw | 25 ml Per VE

Bestelnr. 0807.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 3-4 weken
Vanaf 6 VE 448,35 €/VE
Vanaf 24 VE 424,76 €/VE

Productdetails


For the isolation of His-tagged proteins under reducing conditions.

Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.

  • Superior recovery rate of pure His-tagged proteins
  • Minimized Nickel leaching
  • Compatible with denaturing and reducing reagents

The matrix of ROTI®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. The tetradentate NTA cross-linker binds his-tagged proteins very efficiently, leading to high recovery rates with minimized nickel bleeding into the eluate.


Gebruiksinstructies

ROTI®Garose-His/Ni NTA-Beads may be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10  mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).



ROTI®Garose-His/Ni NTA-Beads ROTI®Garose for biochemistry

Nickel charged NTA-agarose beads for affinity chromatography under reducing conditions.
50 % bead suspension in 20 % ethanol.

  • Rapid one-step purification of very pure His-tagged proteins from total lysates
  • High binding capacity for 6xHis-tagged protein
  • Reliably elution and regeneration
  • For batch mode and gravity flow

Geschikt voor

The matrix of choice if His-tagged proteins of very small amount shall be isolated from reducing buffer solutions without loss.


Gebruiksinstructies

May be autoclaved at 121 °C for 30 mins.



Technische informatie
Gebruikstip Bead suspension, binding capacity ≥50 mg/ml packed matrix (His)6 
ROTI®Garose-His/Ni NTA-Beads
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
0807.1 25 ml plastic

471,95 €

0807.2 100 ml plastic

1.531,90 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)6 %
LigandNTA
Metal ionnickel
Binding capacity (gel) (GFPuv-6xHis)≥ 50 mg/ml
Ethanol15 %