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ROTI®Garose-His/Ni NTA-HPBeads, 25 ml
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170
For isolation of His-tagged proteins by fast or large scale affinity chromatography under reducing conditions
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
596,65 €/VE
Excl. btw | 25 ml Per VE
Bestelnr. 0805.1
Geschikt voor / Toebehoren
Productdetails
For the isolation of His-tagged proteins under reducing conditions.
Immobilized metal ion affinity chromatography (IMAC) still is the most widely used method to obtain high yield of very pure proteins with reasonable effort.
ROTI®Garose His/Ni Beads and ready-to-use columns offer the perfect solution for your specific applications in the field of benchtop column chromatography, for protein isolation in batch processes or under medium pressures (MPLC, FPLC). Depending on the product, our beads are optimally suited for low or high flow rates or when small or large sample volumes are to be purified.
- Superior recovery rate of pure His-tagged proteins
- Minimized Nickel leaching
- Compatible with denaturing and reducing reagents
The matrix of ROTI®Garose-His/Ni NTA products consists of crosslinked and beaded 6 % agarose, NTA-conjugated and charged with divalent Nickel ions. Nickel chelates recognize two histidines that are in close proximity and accessible with good specificity and very high affinity, making the Ni2+ charged matrix the first choice for all standard applications. The tetradentate NTA crosslinker enables highly efficient binding of His-tagged proteins and leads to high recovery rates with minimized nickel bleeding into the eluate.
ROTI®Garose-His/Ni NTA-Beads may be regenerated, making them very cost-effective.
The Matrix is stable in all commonly used reagents including denaturing reagents (like 8 M urea, 6 M guanidinium hydrochloride) and (dependent on the respective buffer) reducing substances (for instance ≤30 mM glutathion, ≤10 mM DTT, ≤10 mM DTE, ≤20 mM β-mercaptoethanol und ≤0,3 % SDS).
ROTI®Garose-His/Ni NTA-HPBeads ROTI®Garose for biochemistry
Nickel charged NTA-agarose beads for affinity chromatography under reducing conditions. The matrix of choice for applications under medium pressure, with high flow rate or with big sample-/matrix volume.
In ROTI®Garose-His/Ni NTA-HPBeads, the advantages of the highly efficient Nickel-His binding have been combined with a bead technology allowing a very rapid flow rate. ROTI®Garose-His/Ni HPBeads are ideal for purifying large sample volumes.
50 % bead suspension in 20 % ethanol.
- Rapid one-step purification of very pure His-tagged proteins from total lysates
- High binding capacity for 6xHis-tagged protein (≥60 mg/ml purified His-tagged protein)
- Reliably elution and regeneration
- For batch mode, gravity flow, MPLC und FPLC
- Recommended for big matrix volumes
MPLC and FPLC, or if His-tag proteins shall be isolated either in particularly short time or in big amounts from reducing solutions.
May be autoclaved at 121 °C for 30 mins.
| Gebruikstip | Bead suspension, binding capacity ≥60 mg/ml packed matrix (His)6 |
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 0805.1 | 25 ml | plastic |
596,65 € |
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| 0805.2 | 100 ml | plastic |
1.929,65 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
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Analysecertificaten
Type analysis
| Particle size | 50-150 µm |
| Beads (cross-linked agarose) | 6 % |
| Ligand | NTA |
| Metal ion | nickel |
| Binding capacity (gel) (GFPuv-6xHis) | ≥60 mg/ml |
| Ethanol | 20 % |