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ROTI®Garose-Protein A Beads, 5 ml

ROTI®Garose for biochemistry
Mogelijke gevolgen: zijn ontvlambaar; vloeistoffen vormen met lucht explosieve mengsels; zorgen met water voor ontvlambare gassen of zijn zelfontvlambaar. Veiligheid: uit de buurt van open vuur en warmtebronnen houden; vaten dicht afsluiten; brandveilig bewaren.
Mogelijke gevolgen: leiden tot schade aan de gezondheid, irriteren organen, huid of luchtwegen. Leiden in grotere hoeveelheden tot de dood. Veiligheid: zoals hierboven; bij huidirritaties of oogcontact met water of geschikt middel spoelen.
Warning
H226-H319
i flammable liquid and vapour, causes serious eye irritation
P210 P280 P305+P351+P338
i keep away from heat. No smoking, wear protective gloves/eye protection, IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing
VE
Verp.
Agarose beads for affinity chromatography, Protein A agarose beads, Column matrix for protein isolation
Density (D) 0,95 g/cm³
Boiling point (bp) >80 °C
Flash point (flp) >50 °C
Storage temp. +4 °C
ADR 3 III
WGK 3
UN-Nr. 1170

For purification of immunoglobulins by affinity chromatography.
For batch mode and gravity flow

For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A and G Beads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
Productdetails

768,65 €/VE 

Excl. btw | 5 ml Per VE

Bestelnr. 1278.1

Beschikbaar
Vrij van verzendingskosten vanaf 250 €
Klaar voor verzending in 3-4 weken
Vanaf 6 VE 730,22 €/VE
Vanaf 24 VE 691,78 €/VE

Productdetails


For rapid and simple purification of antibodies with reasonable effort, affinity chromatography still is the first choice in most cases. ROTI®Garose-Protein A and G Beads offer the perfect solution for your specific application under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.

  • Rapid one-step purification of immunoglobulins from cell lysates and biological solutions
  • High yield of very pure immunoglobulins
  • Easy elution and regeneration
  • Covalent binding of protein G in high ligand density

The matrix of ROTI®Garose-Protein A and G Beads consists of crosslinked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus and protein G from Streptococcus, respectively.
The recombinant protein A (recA) shares IgG binding properties with natural protein A of S. aureus Cowan strain I. It has high affinity for IgG from a variety of different mammalian species, also binding some populations of IgA and IgM.
Binding domains specific for albumin as well as cell walls and cell membranes have been removed in the recombinant protein G, in order to ensure the maximum specific IgG binding capacity. It has high affinity for IgG from a variety of different mammalian species, also binding IgG3 with high affinity.


Gebruiksinstructies

Most immunoglobulins may be eluted in 100 mM glycin or citric acid buffer (pH 3.0). ROTI®Garose-Protein A and G Beads may be regenerated, making them very cost-effective.
May not be autoclaved.



ROTI®Garose-Protein A Beads ROTI®Garose for biochemistry

Protein A coated agarose beads for low pressure affinity chromatography. High-performance affinity resin for antibody purification.
50 % bead suspension in 20 % ethanol.

  • Binding capacity (human IgG) of approx. 25 mg per ml matrix
  • Superior affinity for IgG from various mammalian species
  • Binding of some populations IgA and IgM
  • For batch mode, gravity flow, MPLC und FPLC

Geschikt voor

Isolation of antibodies, in affinity column chromatography or in batch procedures.


Gebruiksinstructies

The matrix of ROTI®Garose Protein A Beads consists of cross-linked and beaded 4 % agarose, coated and covalently coupled with protein A from Staphylococcus aureus.
High binding affinity to IgGs of many different mammalian species, binding of some populations IgA and IgM.



Technische informatie
Gebruikstip Bead suspension, binding capacity ca. 25 mg/ml Suspension (human IgG) 
ROTI®Garose-Protein A Beads
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Prijs Hoeveelheid
1278.1 5 ml plastic

768,65 €

1278.2 25 ml plastic

2.703,65 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Particle size50-150 µm
Beads (cross-linked agarose)4 %
Ligandrecombinant protein A
Chemical bondingcovalent
Ligand density (Protein A)ca. 3 mg/ml
Binding capacity (humane IgG)ca. 25 mg/ml
Stabilizer20 % ethanol