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Bovine Serum Albumin (BSA) Fraction V, pH 5.2, Europe, 200 g
Storage temp. +4 °C
WGK 1
CAS No. 9048-46-8
EG-Nr. 232-936-2
Non-neutralized albumin. Suitable as protein standard or hapten carrier, recommmended for use in ELISA and Western-Blotting.
504,20 €/VE
Excl. btw | 200 g Per VE
Bestelnr. 2834.3
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Productdetails
For purification of this Albumin an extensive heat-shock/diafiltration method is used, which has been shown to bring more highly purified products than the standard process acc. to Cohn. The process is taking place in a closed system.
Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
Bovine Serum Albumin (BSA) Fraction V, pH 5.2, Europe >97 %
Albumin pH 5.2 is basically suitable for all applications using albumins, e.g. for stabilisation of enzymes and antibodies, or as hapten carrier in immunoassays.
The pH value of albumin pH 5.2 is close to its pI and the protein is only weakly charged. It is, therefore, particularly recommended for use in ELISA and Western blotting, since it may help in further reducing background signals.
| Gebruikstip | for protein analysis |
- Tussentotaal: 0.00
| Bestelnr. | VE | Verp. | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 2834.1 | 10 g | plastic |
53,25 € |
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| 2834.2 | 50 g | plastic |
163,95 € |
|
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| 2834.3 | 200 g | plastic |
504,20 € |
|
|
| 2834.4 | 500 g | plastic |
1.069,65 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Molar extinction coefficient: 4,4x104 L x mol-1 x cm-1 (acc. to 0,667 L x mol-1 x cm-1 for a 0,1 % solution).
BSA is a soluble monomeric protein with a molecular weight of 66.5 kDa. It is synthesized in the liver and extracted from the blood of cattle. In the bloodstream it serves as a binding and transport protein. BSA is used as a standard in protein quantification, as a blocking reagent, as a satabilizer in molecular applications and as a nutrient additive in cell cultures.
Purification process
The purification of the BSA is carried out using a far-reaching heat shock/diafiltration method that produces higher purity albumins than the usual Cohn crystallization. The process takes place in a closed system.
Albumin, NZ-Origin is particularly recommended when procedural regulations require the use of albumin of guaranteed origin Australia or New Zealand.
BSA, US-Origin is particularly recommended if the use of albumin with certified US-Origin is dictated by process regulations.
Albumin of European origin is produced from blood of European cattle herds. It is excellent for use in all assays and products manufactured for regions outside the USA, Australia and New Zealand.
Analysecertificaten
Guarantee analysis
| Appearance | flakes |
| Colour | pale yellow to greenish |
| Assay | >97 % |
| Protein | >92 % |
| Dry extract | >95 % |
| pH value (10 % solution) | 5.2 ±0.4 |
| Origin | Europe |