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Denhardt's solution, lyophilised powder

50x, BioScience Grade, for molecular biology
VE
Verp.
Verpakking
Storage temp. +4 °C
WGK 3

Blocking reagent for hybridisation and stripping.
The bottle contains powder mix for 50 ml 50x stock solution.Add 48,8 ml water to gain exactly 50 ml stock solution. Sterilise stock solution by filtration (0,2 µm) and store in aliquots of 10 ml at -20 °C .

Denhardt's lyophilised powder contains a mixture of different blocking agents that is used for nucle ...
Productdetails
Type analysis

103,75 €/VE 

Excl. btw | 50 ml Per VE

Bestelnr. HP33.1

Op voorraad
Vrij van verzendingskosten vanaf 125 €
Snelle en betrouwbare levering!
Vanaf 6 VE 98,56 €/VE
Vanaf 24 VE 93,38 €/VE
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Denhardt's solution, lyophilised powder 50x, BioScience Grade, for molecular biology

Denhardt's lyophilised powder contains a mixture of different blocking agents that is used for nuclear acid hybridisation (Northern and Southern Blot) as well as for stripping of hybridised membranes. By prehybridisation the filter membranes with Denhardt's solution unspecific binding of DNA to the membrane is avoided.


Gebruiksinstructies

For hybridisation use 10x Denhardt's solution with 6x SSC (ROTI®Stock 20x SSC, Art. No. 1054.1) supplemented with 100 µg/ml denatured salmon sperm DNA and 1 % SDS (ROTI®Stock 20 % SDS, Art. No. 1057.1).



Denhardt's solution, lyophilised powder
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
HP33.1 50 ml plastic Powder for 50 ml 103,75 €
Op voorraad
Binnenkort beschikbaar
Niet beschikbaar
Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

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Type analysis

Albumin Fraction V0,5 g  
Ficoll 4000,5 g  
Polyvinylpyrrolidone K300,5 g  
pH-value (stock solution in water)6,3 ±0,2  

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