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T831_Rolle 3A7E_Button_rz Membran_Demo

Transfer membrane ROTI® ProBind PVDF 0.2, 10 × 10 cm
Van  Carl ROTH
Icon_ready-to-use Ready-to-use products and reagents
Icon_Muster-verfuegbar For inquiries and further information, please contact our sales team
Materiaal: polyvinylidenfluoride (PVDF).
Verwante producten
Afmeting


Transfer Membrane for Nucleic Acid and Protein Immobilization Techniques

ProBind transfer membranes are the ideal choice for reliable protein analysis, Southern and Northern blots. Available as PVDF or nitrocellulose (NC) membranes, they offer high mechanical stability, excellent binding capacity, and minimal background interference. Their uniform structure ensures sharp and clear bands for precise results.Thanks to their high chemical resistance and optimal sensitivity, ProBind transfer membranes are perfect for a wide range of detection methods—from classic blotting techniques to advanced assays.
Productdetails

301,00 €/VE 

Excl. btw | 25 blad Per VE

Bestelnr. 3A7H.1

Op voorraad
Snelle en betrouwbare levering!
Vrij van verzendingskosten vanaf 250 €
Vanaf 6 VE 285,95 €/VE
Vanaf 24 VE 270,90 €/VE

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3A85
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Blotting-Sandwich ROTI® ProBlot PVDF 0.45
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Productdetails

Materiaal: polyvinylidenfluoride (PVDF).

Transfer Membrane for Nucleic Acid and Protein Immobilization Techniques

ProBind transfer membranes are the ideal choice for reliable protein analysis, Southern and Northern blots. Available as PVDF or nitrocellulose (NC) membranes, they offer high mechanical stability, excellent binding capacity, and minimal background interference. Their uniform structure ensures sharp and clear bands for precise results.Thanks to their high chemical resistance and optimal sensitivity, ProBind transfer membranes are perfect for a wide range of detection methods—from classic blotting techniques to advanced assays.



Transfer membrane ROTI® ProBind PVDF 0.2 

Toepassingsgebieden:
  • Western Blotting
  • Southern Blotting
  • Northern Blotting
  • Amino Acid and Protein analysis
3A7H_Demo


Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Afmeting 10 × 10 cm 
Uitvoering Sheet 
Poriëngr. 0,2 µm
Binding hydrophobic 
Dikte 150±20 µm
Immobilisering not necessary 
Bindingscapaciteit ≥260 µg/cm²
Transfer membrane ROTI® ProBind PVDF 0.2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Afmeting VE Prijs Hoeveelheid
3A7H.1 10 × 10 cm 25 blad

301,00 €

3A7K.1 300 × 30 cm 1 rol(len)

548,25 €

Op voorraad
Beschikbaar (let op levertijd)
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Geen documenten beschikbaar

Algemene informatie

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not