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Transfer membrane ROTI®NC 0.45, 400 × 30 cm
High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
492,35 €/VE
Excl. btw | 1 rol(len) Per VE
Bestelnr. 9201.1
Geschikt voor / Toebehoren
Productdetails
Transfer membrane for protein analyses, Southern- and Northern-Blots.
High-quality transfer membrane for all detection systems - extremely suitable for all protein transfer and detection methods. The membrane stands out for its particularly high mechanical stability. The pure 100 % nitrocellulose quality membrane is easy to block and allows for background-free blots with perfect bands.
Transfer membrane ROTI®NC 0.45
Afmeting | 400 × 30 cm |
Uitvoering | roll |
Binding | hydrophobic |
Bindingscapaciteit | 100 µg/cm² |
Immobilisering | UV, baking (max. 80 °C) |
- Tussentotaal: 0.00
Bestelnr. | Afmeting | VE | Prijs | Hoeveelheid | |
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9201.1 | 400 × 30 cm | 1 rol(len) |
492,35 € |
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9200.1 | 300 × 30 cm | 1 rol(len) |
385,95 € |
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Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
Technical Parameters of ROTH-Transfer Membranes
Membrane | Material | Characteristics | Thickness (μm) | Binding capacity (μg/cm2) | Pore size (μm) | Immobilisation |
Roti® Fluoro PVDF | Polyvinyliden fluoride | hydrophobic | 125 ±25 | 300 | 0,2 | not necessary |
Transfer membrane ROTI®PVDF 0.2 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,2 | not necessary |
Transfer membrane ROTI®PVDF 0.45 | Polyvinyliden fluoride | hydrophobic | 190 ±50 | ≥125 | 0,45 | not necessary |
Immobilon P | Polyvinyliden fluoride | hydrophobic | 120 ±25 | 150-300 | 0,45 | not necessary |
Roti® Nylon 0.2 | Nylon 6,6 | neutral/amphoteric | 150 ±20 | 500 | 0,2/0,45 | UV, baking |
Roti® Nylon plus | Nylon 6,6 | hydrophilic, positively charged | 160 ±20 | 500 | 0,45 | not necessary, UV and baking possible |
Transfer membrane ROTI®NC 0.2 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,2 | UV, baking (max. 80 °C) |
Transfer membrane ROTI®NC 0.45 | Nitrocellulose | hydrophobic | 150 ±50 | 100 | 0,45 | UV, baking (max. 80 °C) |
Amersham Protran® | Nitrocellulose | electrostatic, hydrophobic | unknown | 11-72 | 0,2/0,45 | UV, baking (max. 80 °C) |
Amersham Protran® supported | Nitrocellulose | electrostatic, hydrophobic | unknown | 86-110 | 0,2/0,45 | UV, baking (max. 80 °C) |
Biomolecules / Membrane Pores | Size (kD) |
genomic DNA (human) | >11000000 |
E. coli | approx. 1500000 |
0.45 μm | approx. 5000 |
0.2 μm | approx. 2000 |
DNA 3 kb | approx. 1000 |
RNA 1 kb | approx. 300 |
Antibodies (IgG) | approx. 150 |
Proteins in the lab | approx. 10–300 |
BSA (Albumin) | 69 |
Biomolecules | Nylon plus | Nylon 0.2 | PVDF 0.2 | PVDF | Fluoro PVDF | NC | NC 0.45 |
DNA | +++ | + | - | - | - | + | + |
RNA | + | +++ | - | - | - | + | + |
Proteins | - | + | +++ | +++ | +++ | + | + |
Detection System | |||||||
Colour | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Radioactivity | +++ | +++ | +++ | +++ | +++ | +++ | +++ |
Chemoluminescence | +++ | +++ | +++ | +++ | - | + | + |
Fluorescence | +++ | +++ | - | - | +++ | - | - |
+++: recommended | +: suitable | -: not |