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Transfer membrane ROTI®Nylon 0.2, Ø13.2 cm

Van  Carl ROTH
Materiaal: nylon 6.6.
Afmeting


342,95 €/VE 

Excl. btw | 50 blad Per VE

Bestelnr. HP39.1

Niet meer verkrijgbaar
Vrij van verzendingskosten vanaf 250 €
Vanaf 6 VE 325,80 €/VE
Vanaf 24 VE 308,66 €/VE

Productdetails

Materiaal: nylon 6.6.

.




Transfer membrane ROTI®Nylon 0.2 

Ideal for DNA/RNA transfer, particularly for colony- and plaque-lifting, as well as for all multiple rehybridisations.
Especially recommended for Northern-blots.


ROTI®Nylon 0.2 is a neutral, enhanced Nylon membrane. Based on the material it provides a superior tensile strength and is extremely resistant against alcohol, acetone, and many other organic solvents.

In a buffer pH of 6,5 ROTI®Nylon 0.2 is uncharged and by means of the surface-standing amino- and carboxy-groups has superior hydrophilic qualities. The charge of the membrane (ζ-potential) may be altered through the pH of the buffer - the membrane is positively charged at pH <6,5 and negatively charged at pH >6,5.
Membrane of particulary high DNA-binding capacity. Available as roll (pore size 0,2 µm) for high-flexible usage, and as disks (pore size 0,45 µm) for colony- and plaque-lifting. May be steam sterilised.
Covalent bondage of transferred nucleic acids is achieved by cross linking (baking for 15 min at 80 °C or UV-cross linking for 20 sec at 260 nm). Due to the excellent signal-to-noise ratio of the membrane, very sensitive detection (single copy-gene detection) and multiple rehybridisation is possible without loosing sensitivity.
Detection systems: radioactivity, chemoluminescence, fluorescence

Toepassingsvoorbeelden:
  • Southern- and Northern blot
  • Dot Blot
  • DNA fingerprinting
  • ELISA
  • colony and plaque lifting
  • Macro- and Microarrays
  • Multiple Rehybridisations


Technische informatie
Afmeting Ø13,2 cm 
Uitvoering disks 
Poriëngr. 0,45 µm
Type 0,45 µm 
Lading neutral/amphoteric 
Bindingscapaciteit 500 µg/cm²
Dikte 150±20 µm
Immobilisering UV, baking 
Transfer membrane ROTI®Nylon 0.2
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Afmeting VE Prijs Hoeveelheid
AE50.1 300 × 30 cm 1 rol(len)

401,00 €

HP39.1 Ø13,2 cm 50 blad

342,95 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Algemene informatie

Technical Parameters of ROTH-Transfer Membranes

Membrane Material Characteristics Thickness (μm) Binding capacity (μg/cm2) Pore size (μm) Immobilisation
Roti® Fluoro PVDF Polyvinyliden fluoride hydrophobic 125 ±25 300 0,2 not necessary
Transfer membrane ROTI®PVDF 0.2 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,2 not necessary
Transfer membrane ROTI®PVDF 0.45 Polyvinyliden fluoride hydrophobic 190 ±50 ≥125 0,45 not necessary
Immobilon P Polyvinyliden fluoride hydrophobic 120 ±25 150-300 0,45 not necessary
Roti® Nylon 0.2 Nylon 6,6 neutral/amphoteric 150 ±20 500 0,2/0,45 UV, baking
Roti® Nylon plus Nylon 6,6 hydrophilic, positively charged 160 ±20 500 0,45 not necessary, UV and baking possible
Transfer membrane ROTI®NC 0.2 Nitrocellulose hydrophobic 150 ±50 100 0,2 UV, baking (max. 80 °C)
Transfer membrane ROTI®NC 0.45 Nitrocellulose hydrophobic 150 ±50 100 0,45 UV, baking (max. 80 °C)
Amersham Protran® Nitrocellulose electrostatic, hydrophobic unknown 11-72 0,2/0,45 UV, baking (max. 80 °C)
Amersham Protran® supported Nitrocellulose electrostatic, hydrophobic unknown 86-110 0,2/0,45 UV, baking (max. 80 °C)

Size Comparison of Membrane Pores and Biomolecules

Biomolecules / Membrane Pores Size (kD)
genomic DNA (human) >11000000
E. coli approx. 1500000
0.45 μm approx. 5000
0.2 μm approx. 2000
DNA 3 kb approx. 1000
RNA 1 kb approx. 300
Antibodies (IgG) approx. 150
Proteins in the lab approx. 10–300
BSA (Albumin) 69

Application of ROTH-Transfer Membranes

Biomolecules Nylon plus Nylon 0.2 PVDF 0.2 PVDF Fluoro PVDF NC NC 0.45
DNA +++ + - - - + +
RNA + +++ - - - + +
Proteins - + +++ +++ +++ + +
Detection System
Colour +++ +++ +++ +++ +++ +++ +++
Radioactivity +++ +++ +++ +++ +++ +++ +++
Chemoluminescence +++ +++ +++ +++ - + +
Fluorescence +++ +++ - - +++ - -
+++: recommended +: suitable -: not

Analysecertificaten

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