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Size Exclusion Chromatography Columns ROTI®Dex-25 Medium Spin

Icon_Steril Sterile products
Icon_ready-to-use Ready-to-use products and reagents
Steriel
Uitvoering
VE
Gel filtration, Gel permeation chromatography (GPC), Dextran-Epichlorhydrin-Copolymer, Size-exclusion chromatography (SEC)
Storage temp. +2 to +25 °C

For desalting and buffer exchange of protein and nucleic acid solutions.
Suitable for proteins >5 kDa and oligonucleotides >10 bp.

Prepacked ROTI®Dex-Spin columns for size exclusion chromatography by centrifugation, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
For preparation of small sample volumes (optimal 50 µl) in less than 5 minutes.
Productdetails
224,70 € /VE

179,70 €/VE  Campaign price!

Excl. btw | 25 stuks Per VE

Bestelnr. 21C4.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!

Productdetails

Steriel

Prepacked ROTI®Dex-Spin columns for size exclusion chromatography by centrifugation, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
For preparation of small sample volumes (optimal 50 µl) in less than 5 minutes.


The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.


In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.

Voordelen:
  • Sample preparation in just a few minutes
  • Desalting, removal of impurities and buffer exchange in a single step
  • No interaction between analyte and medium
  • High selectivity, high resolution and high yield (> 95%)
  • Also suitable for small sample volumes
  • High chemical stability and therefore compatible with many organic and other solvents


Size Exclusion Chromatography Columns ROTI®Dex-25 Medium Spin 

The column bed consists of ROTI®Dex-25 Medium (Art. No. 21A5), pre-swollen in deionised water.

Geschikt voor:
  • Oligonucleotides > 10 bp
  • Globular proteins > 5 kDa
  • Particles > 2 nm

For optimal results, a sample volume of 50 µl is recommended.


Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.



Technische informatie
Uitvoering ROTI®Dex-25 Spin 
Matrix Cross-linked dextran 
MWCO 5 kDa
10 bp
Partikel grootte Dry: 50-150 µm
Wet: 85-260 µm
Lageringsbuffer Deionized water 
Monsterinhoud 0.002 - 0.1 (optimal 0,05) ml
Size Exclusion Chromatography Columns ROTI®Dex-25 Medium Spin
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Uitvoering VE Prijs Hoeveelheid
21C4.1 ROTI®Dex-25 Spin 25 stuks
224,70 €

179,70 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS

Algemene informatie

U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


The ROTI®Dex ready-to-use columns at a glance

Application Order No. Matrix Particle size (wet) Gel bed volume Sample volume MWCO Pack Qty.
Spin 21C4.1 ROTI®Dex 25 Medium 85-260 µm 0.5 ml 2-100 µl (50 µl optimal) Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
25 Pcs
21C5.1 ROTI®Dex 50 Medium 100-300 µm 0.5 ml 2-100 µl (50 µl optimal) Proteins >25 kDa,
Oligonucleotides >20 bp,
Nanoparticles >4 nm
25 Pcs
Grav 21AX.1 ROTI®Dex 25 Medium 85-260 µm 1.78 ml 0.15-0.3 ml Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
50 Pcs
21AY.1 2.75 ml 0.5 ml 50 Pcs
21C0.1 4.31 ml 1 ml 50 Pcs
21C1.1 10.37 ml 2.5 ml 25 Pcs
21C2.1 17.2 ml 5 ml 10 Pcs
21C3.1 34.21 ml 10 ml 10 Pcs
FPLC 21C6.1 ROTI®Dex 25 Superfine 40-110 μm 1 ml 0.05-0.3 ml Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
5 Pcs
21C6.2 1 ml 0.05-0.3 ml 100 Pcs
21C7.1 5 ml 0.1-1.5 ml 5 Pcs
21C7.2 5 ml 0.1-1.5 ml 25 Pcs
21C7.3 5 ml 0.1-1.5 ml 100 Pcs (4x25)

Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Dextran Blue recovery yield (Yield of Dextran blue in eluate from a 50 µL solution of 1 mg/mL Dextran Blue application)≥70 %
Fluorescein Removal (Fluorescence in the eluate from a 1mM 5/6-carboxyfluorescein in 0.1 M NaHCO3 application)≤0.01 %