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Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 1 ml, 5 stuks

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Warning
H226
i flammable liquid and vapour
P210 P501
i keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking, dispose of contents/container in accordance with local/regional/national/international regulations
Steriel
Uitvoering
VE
Gel filtration, Gel permeation chromatography (GPC), Dextran-Epichlorhydrin-Copolymer, Size-exclusion chromatography (SEC)
Density (D) ~1 g/cm³
Flash point (flp) 43 °C
Storage temp. +2 to +25 °C
WGK 2

For desalting and buffer exchange of protein and nucleic acid solutions.
Suitable for proteins >5 kDa and oligonucleotides >10 bp.

Prepacked ROTI®Dex-FPLC columns for size exclusion chromatography using syringe, pump or FPLC systems, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
Productdetails

428,95 €/VE 

Excl. btw | 5 stuks Per VE

Bestelnr. 21C6.1

Op voorraad
Vrij van verzendingskosten vanaf 250 €
Snelle en betrouwbare levering!
Vanaf 6 VE 407,50 €/VE
Vanaf 24 VE 386,06 €/VE

Productdetails

Steriel

Prepacked ROTI®Dex-FPLC columns for size exclusion chromatography using syringe, pump or FPLC systems, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.

Geschikt voor:
  • 'Fast Protein Liquid Chromatography' (FPLC)
  • Common chromatographs such as ÄKTA™FPLC™
  • Syringe and peristaltic pumping systems

The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.


In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.

Voordelen:
  • Sample preparation in just a few minutes
  • Desalting, removal of impurities and buffer exchange in a single step
  • No interaction between analyte and medium
  • High selectivity, high resolution and high yield (> 95%)
  • Also suitable for small sample volumes
  • High chemical stability and therefore compatible with many organic and other solvents


Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 1 ml 

The column bed consists of ROTI®Dex-25 Superfine. It is the gel filtration medium with the smallest dextran particles, which provide efficient results and very high resolutions. Biomolecules >5 kDa or >10 bp and particles >2 nm are purified. The fractionation range for proteins/peptides/biomolecules is between 1 and 5 kDa, with the highest resolution (most efficient separation) achieved between biomolecules >5 kDa and <1 kDa.

Geschikt voor:
  • Oligonucleotides > 10 bp
  • Globular proteins > 5 kDa
  • Particles > 2 nm

Sample volumes of 0.05 - 0.3 ml are recommended for the 1 ml columns.


Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.



Technische informatie
Uitvoering 1 ml bed volume 
Matrix Cross-linked dextran 
MWCO 5 kDa
10 bp
Partikel grootte Dry: 20-50 µm
Wet: 40-110 µm
Lageringsbuffer 20 % (v/v) ethanol 
Monsterinhoud 0.05 – 0.3 ml
Druk max. 3 bar (0.3 MPa) 
aanbevolen doorstromingswaarde 0.5 - 2 ml/min
maximale doorstromingswaarde 3 ml/min
Zuilvorm 0.7 cm inner diameter x 2.5 cm height 
Aansluiting ingang 10–32 (1/16”) female 
Aansluiting uitgang 10–32 (1/16”) male 
Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 1 ml
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. Uitvoering VE Prijs Hoeveelheid
21C6.1 1 ml bed volume 5 stuks

428,95 €

21C6.2 1 ml bed volume 100 stuks

7.519,65 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!


The ROTI®Dex ready-to-use columns at a glance

Application Order No. Matrix Particle size (wet) Gel bed volume Sample volume MWCO Pack Qty.
Spin 21C4.1 ROTI®Dex 25 Medium 85-260 µm 0.5 ml 2-100 µl (50 µl optimal) Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
25 Pcs
21C5.1 ROTI®Dex 50 Medium 100-300 µm 0.5 ml 2-100 µl (50 µl optimal) Proteins >25 kDa,
Oligonucleotides >20 bp,
Nanoparticles >4 nm
25 Pcs
Grav 21AX.1 ROTI®Dex 25 Medium 85-260 µm 1.78 ml 0.15-0.3 ml Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
50 Pcs
21AY.1 2.75 ml 0.5 ml 50 Pcs
21C0.1 4.31 ml 1 ml 50 Pcs
21C1.1 10.37 ml 2.5 ml 25 Pcs
21C2.1 17.2 ml 5 ml 10 Pcs
21C3.1 34.21 ml 10 ml 10 Pcs
FPLC 21C6.1 ROTI®Dex 25 Superfine 40-110 μm 1 ml 0.05-0.3 ml Proteins >5 kDa,
Oligonucleotides >10 bp,
Nanoparticles >2 nm
5 Pcs
21C6.2 1 ml 0.05-0.3 ml 100 Pcs
21C7.1 5 ml 0.1-1.5 ml 5 Pcs
21C7.2 5 ml 0.1-1.5 ml 25 Pcs
21C7.3 5 ml 0.1-1.5 ml 100 Pcs (4x25)

Technical data of the ROTI®Dex FPLC columns at a glance:

Order No. 21C6.1 21C6.2 21C7.1 21C7.2 21C7.3
Package size 5 Pcs 100 Pcs 5 Pcs 25 Pcs 100 Pcs
Matrix ROTI®Dex 25 Superfine
Particle size (wet) 40-110 μm
Gel bed volume 1 ml 5 ml
Sample volume 0.05-0.3 ml 0.1-1.5 ml
Pressure max. 3 bar (0.3 MPa)
Flow rate max. 3 ml/min 10 ml/min
Recommended flow rate 0.5-2 ml/min 1-5 ml/min
Outlet port 10-32 (1/16″) male
Inlet port 10-32 (1/16″) female
Column size 0.7 cm inner diameter x 2.5 cm height 1.6 cm inner diameter x 2.5 cm height

Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Packing evaluation Asymmetry: (5 % CV 2M NaCl salt plug; flow rate 1 mL/min, run in PBS)0.7 ≤ As ≤ 1.6
Overlap of peak areas: (20 % CV (0.2 mL) 2 g/L BSA and 2 M NaCl; flow rate 1 mL/min, run in PBS)≤10 %