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Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 5 ml, 5 stuks
Ready-to-use products and reagents
For inquiries and further information, please contact our sales team
Flash point (flp) 43 °C
Storage temp. +2 to +25 °C
WGK 2
For desalting and buffer exchange of protein and nucleic acid solutions.
317,15 €/VE
Excl. btw | 5 stuks Per VE
Bestelnr. 21C7.1
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Productdetails
Prepacked ROTI®Dex-FPLC columns for size exclusion chromatography using syringe, pump or FPLC systems, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
- 'Fast Protein Liquid Chromatography' (FPLC)
- Common chromatographs such as ÄKTA™FPLC™
- Syringe and peristaltic pumping systems
The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
Size Exclusion Chromatography Columns ROTI®Dex-25 Superfine FPLC 5 ml
The column bed consists of ROTI®Dex-25 Superfine. It is the gel filtration medium with the smallest dextran particles, which provide efficient results and very high resolutions. Biomolecules >5 kDa or >10 bp and particles >2 nm are purified. The fractionation range for proteins/peptides/biomolecules is between 1 and 5 kDa, with the highest resolution (most efficient separation) achieved between biomolecules >5 kDa and <1 kDa.
- Oligonucleotides > 10 bp
- Globular proteins > 5 kDa
- Particles > 2 nm
Sample volumes of 0.1-1.5 ml are recommended for the 5 ml columns.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Uitvoering | 5 ml bed volume |
| Matrix | Cross-linked dextran |
| MWCO |
5 kDa 10 bp |
| Partikel grootte |
Dry: 20-50 µm Wet: 40-110 µm |
| Lageringsbuffer | 20 % (v/v) ethanol |
| Monsterinhoud | 0.1 – 1.5 ml |
| Druk max. | 3 bar (0.3 MPa) |
| aanbevolen doorstromingswaarde | 1 - 5 ml/min |
| maximale doorstromingswaarde | 10 ml/min |
| Zuilvorm | 1.6 cm inner diameter x 2.5 cm height |
| Aansluiting ingang | 10–32 (1/16”) female |
| Aansluiting uitgang | 10–32 (1/16”) male |
- Tussentotaal: 0.00
| Bestelnr. | Uitvoering | VE | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 21C7.1 | 5 ml bed volume | 5 stuks |
317,15 € |
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| 21C7.2 | 5 ml bed volume | 25 stuks |
2.311,25 € |
|
|
| 21C7.3 | 5 ml bed volume | 100 stuks |
4.998,75 € |
|
|
|
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!
The ROTI®Dex ready-to-use columns at a glance
| Application | Order No. | Matrix | Particle size (wet) | Gel bed volume | Sample volume | MWCO | Pack Qty. |
| Spin | 21C4.1 | ROTI®Dex 25 Medium | 85-260 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
25 Pcs |
| 21C5.1 | ROTI®Dex 50 Medium | 100-300 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >25 kDa, Oligonucleotides >20 bp, Nanoparticles >4 nm |
25 Pcs | |
| Grav | 21AX.1 | ROTI®Dex 25 Medium | 85-260 µm | 1.78 ml | 0.15-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
50 Pcs |
| 21AY.1 | 2.75 ml | 0.5 ml | 50 Pcs | ||||
| 21C0.1 | 4.31 ml | 1 ml | 50 Pcs | ||||
| 21C1.1 | 10.37 ml | 2.5 ml | 25 Pcs | ||||
| 21C2.1 | 17.2 ml | 5 ml | 10 Pcs | ||||
| 21C3.1 | 34.21 ml | 10 ml | 10 Pcs | ||||
| FPLC | 21C6.1 | ROTI®Dex 25 Superfine | 40-110 μm | 1 ml | 0.05-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
5 Pcs |
| 21C6.2 | 1 ml | 0.05-0.3 ml | 100 Pcs | ||||
| 21C7.1 | 5 ml | 0.1-1.5 ml | 5 Pcs | ||||
| 21C7.2 | 5 ml | 0.1-1.5 ml | 25 Pcs | ||||
| 21C7.3 | 5 ml | 0.1-1.5 ml | 100 Pcs (4x25) |
Technical data of the ROTI®Dex FPLC columns at a glance:
| Order No. | 21C6.1 | 21C6.2 | 21C7.1 | 21C7.2 | 21C7.3 |
| Package size | 5 Pcs | 100 Pcs | 5 Pcs | 25 Pcs | 100 Pcs |
| Matrix | ROTI®Dex 25 Superfine | ||||
| Particle size (wet) | 40-110 μm | ||||
| Gel bed volume | 1 ml | 5 ml | |||
| Sample volume | 0.05-0.3 ml | 0.1-1.5 ml | |||
| Pressure max. | 3 bar (0.3 MPa) | ||||
| Flow rate max. | 3 ml/min | 10 ml/min | |||
| Recommended flow rate | 0.5-2 ml/min | 1-5 ml/min | |||
| Outlet port | 10-32 (1/16″) male | ||||
| Inlet port | 10-32 (1/16″) female | ||||
| Column size | 0.7 cm inner diameter x 2.5 cm height | 1.6 cm inner diameter x 2.5 cm height | |||
Analysecertificaten
Type analysis
| Packing evaluation (5% CV 2M NaCl salt plug, flow rate 5 mL/min, run in PBS) | 0.7 ≤ As ≤ 1.9 |
| Overlap of peak areas: (20 % CV (0.2 mL) 2 g/L BSA and 2 M NaCl; flow rate 1 mL/min, run in PBS) | ≤5 % |