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Size Exclusion Chromatography Columns ROTI®Dex-50 Medium Spin
Sterile products
Ready-to-use products and reagents
For desalting and buffer exchange of protein and nucleic acid solutions.
For preparation of small sample volumes (optimal 50 µl) in less than 5 minutes.
107,50 €/VE Campaign price!
Excl. btw | 25 stuks Per VE
Bestelnr. 21C5.1
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Productdetails
Prepacked ROTI®Dex-Spin columns for size exclusion chromatography by centrifugation, for fast and efficient removal of unwanted low molecular weight factors, such as impurities, salts, excipients, markers, cell debris, etc. from protein or nucleic acid solutions.
For preparation of small sample volumes (optimal 50 µl) in less than 5 minutes.
The purification is based on a spherical porous gel filtration medium consisting of dextran cross-linked with epichlorohydrin.
In size exclusion chromatography molecules are separated according to their size in solution. Smaller molecules pass through the column much slower than larger molecules.
In contrast to other chromatography methods, no interactions occur here between the analyte and the stationary phase of the column. This provides the ideal basis for the purification and analysis of intact proteins.
- Sample preparation in just a few minutes
- Desalting, removal of impurities and buffer exchange in a single step
- No interaction between analyte and medium
- High selectivity, high resolution and high yield (> 95%)
- Also suitable for small sample volumes
- High chemical stability and therefore compatible with many organic and other solvents
Size Exclusion Chromatography Columns ROTI®Dex-50 Medium Spin
The column bed consists of ROTI®Dex-50 Medium (Art. No. 21A6), pre-swollen in deionised water.
- Oligonucleotides > 20 bp
- Globular proteins > 25 kDa
- Particles > 4 nm
For optimal results, a sample volume of 50 µl is recommended.
Detailed information on the application can be found in our Technical Information Brochure and in our Instructions for Use.
| Uitvoering | ROTI®Dex-50 Spin |
| Matrix | Cross-linked dextran |
| MWCO |
25 kDa 20 bp |
| Partikel grootte |
Dry: 50-150 µm Wet: 100-300 µm |
| Lageringsbuffer | Deionized water |
| Monsterinhoud | 0.002 - 0.1 (optimal 0,05) ml |
- Tussentotaal: 0.00
| Bestelnr. | Uitvoering | VE | Prijs | Hoeveelheid | |
|---|---|---|---|---|---|
| 21C5.1 | ROTI®Dex-50 Spin | 25 stuks |
134,40 €
107,50 € |
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- Tussentotaal: 0.00
Downloads / MSDS
Algemene informatie
U vindt nog meer aantrekkelijke aanbiedingen om uw chromatografielaboratorium te vervolledigen op onze Chromatografie pagina!
The ROTI®Dex ready-to-use columns at a glance
| Application | Order No. | Matrix | Particle size (wet) | Gel bed volume | Sample volume | MWCO | Pack Qty. |
| Spin | 21C4.1 | ROTI®Dex 25 Medium | 85-260 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
25 Pcs |
| 21C5.1 | ROTI®Dex 50 Medium | 100-300 µm | 0.5 ml | 2-100 µl (50 µl optimal) | Proteins >25 kDa, Oligonucleotides >20 bp, Nanoparticles >4 nm |
25 Pcs | |
| Grav | 21AX.1 | ROTI®Dex 25 Medium | 85-260 µm | 1.78 ml | 0.15-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
50 Pcs |
| 21AY.1 | 2.75 ml | 0.5 ml | 50 Pcs | ||||
| 21C0.1 | 4.31 ml | 1 ml | 50 Pcs | ||||
| 21C1.1 | 10.37 ml | 2.5 ml | 25 Pcs | ||||
| 21C2.1 | 17.2 ml | 5 ml | 10 Pcs | ||||
| 21C3.1 | 34.21 ml | 10 ml | 10 Pcs | ||||
| FPLC | 21C6.1 | ROTI®Dex 25 Superfine | 40-110 μm | 1 ml | 0.05-0.3 ml | Proteins >5 kDa, Oligonucleotides >10 bp, Nanoparticles >2 nm |
5 Pcs |
| 21C6.2 | 1 ml | 0.05-0.3 ml | 100 Pcs | ||||
| 21C7.1 | 5 ml | 0.1-1.5 ml | 5 Pcs | ||||
| 21C7.2 | 5 ml | 0.1-1.5 ml | 25 Pcs | ||||
| 21C7.3 | 5 ml | 0.1-1.5 ml | 100 Pcs (4x25) |
Analysecertificaten
Type analysis
| Dextran Blue recovery yield (Yield of Dextran blue in eluate from a 50 µL solution of 1 mg/mL Dextran Blue application) | ≥70 % |
| Fluorescein Removal (Fluorescence in the eluate from a 1mM 5/6-carboxyfluorescein in 0.1 M NaHCO3 application) | ≤0.001 % |