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DEAE Dextran, 100 g

for biochemistry
VE
Verp.
Diethylaminoethyldextran
Molar mass (M) ~500000 g/mol
WGK 1
CAS No. [9013-34-7]

Transfection reagent

DEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethy ...
Productdetails
Type analysis

190,85 €/VE 

Excl. btw | 100 g Per VE

Bestelnr. 4198.2

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Vanaf 6 VE 181,31 €/VE
Vanaf 24 VE 171,76 €/VE
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Productdetails



DEAE Dextran for biochemistry

DEAE is the oldest transfection reagent known. The method of complexation of plasmid DNA with diethylaminoethyldextran has been published in 1968 by McCutchan. Positively charged DEAE binds negatively charged DNA to aggregates, which then bind to negtively charged surface structures of cell membranes. Uptake is done via endocytosis. This DEAE method needs only little amounts of DNA, while resulting in high transfection efficiency of up to 30 %. However, the cytotoxic effects caused by DEAE allow only transient transfections. The following cell lines are well transfectable: CV-1, BSC-1 and COS cells, and other stabile, insensitive cell lines.

Stock solution: 50 mg/ml in H2O. Autoclave.

End concentration: 1 mg/ml (transfection for 30-90 min.) or 250 µg/ml (transfection for up to 8 h)



DEAE Dextran
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Bestelnr. VE Verp. Prijs Hoeveelheid
4198.1 25 g plastic 60,10 €
4198.2 100 g plastic 190,85 €
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Leveringsdatum onbekend op dit moment
Geselecteerde hoeveelheid:   0
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Analysecertificaten

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De volgende analysecertificaten werden gevonden:

Type analysis

Appearancewhite to creamcoloured powder  
Loss on drying≤10 %  
pH value (5 % in water)5,0-7,0  
Nitrogen content (N)3,0-3,8 %  
Sulphated ash≤2 %  
Free DEAE-Cl≤0,002 %  

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