Naar de inhoud springen Naar het navigatiemenu springen

ROTI®Pol ProofRead, 40 µl, 1 x 40 µl

5 U/μl
Icon_Eisstern All products identified as being particularly temperaturesensitive are shipped in special ice boxes with freezer packs or in dry ice.
VE
Verp.
Verpakking
DNA polymerase (thermostable), Pfu DNA polymerase, recombinant, Polymerase (thermostable)
Storage temp. -20 °C
Transport temp. cooled
WGK 1

For high fidelity PCR amplifications.
Proof read polymerase in storage buffer, proof read PCR buffer (10x)

For PCR. Recombinant, heat stable DNA polymerase from the thermophilic bacterium Thermus aquaticus or Pyrococcus furiosus.
Productdetails

102,70 €/VE 

Excl. btw | 40 µl Per VE

Bestelnr. 9344.1

In productie
Vrij van verzendingskosten vanaf 250 €
Vanaf 6 VE 97,56 €/VE
Vanaf 24 VE 92,43 €/VE

Productdetails


For PCR. Recombinant, heat stable DNA polymerase from the thermophilic bacterium Thermus aquaticus or Pyrococcus furiosus.


ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.



ROTI®Pol ProofRead 5 U/μl

Recombinant version of the heat stable Pfu DNA polymerase from the extreme thermophilic archae bacterium Pyrococcus furiosus in storage buffer, plus additional 10x concentrated PCR-ProofRead reaction buffer. ROTI®Pol ProofRead is recommended for use in all PCR applications in which exact sequence fidelity is required.

This polymerase set ROTI®Pol ProofRead is the optimal choice for all high fidelity PCR assays, as being performed, for instance, prior to cloning or sequencing, in cycle sequencing or in site-directed mutagenesis reactions. In combination with the attached specially adjusted buffer, the ProofRead polymerase delivers sequence identical PCR amplificates of good yield with a wide range of PCR templates. Additionally, ROTI®Pol ProofRead is well suited for amplification of target sequences of high GC content, or with complex secondary structures.

ROTI®Pol ProofRead is able to amplify PCR products up to 3 kb with genomic DNA and is appropriate for use in the amplification of a broad variety of template DNAs. The ProofRead DNA polymerase included in the set replicates DNA 5’ → 3’ at 72 °C to 75 °C under presence of magnesium ions. Furthermore, the ProofRead-DNA polymerase possesses a 3’ → 5’ (proof reading) exonuclease activity, rapidly substituting misincorporated bases during polymerization, and thus being responsible for the high sequence fidelity. ROTI®Pol ProofRead generated DNA fragments are blunt-ended.


Filled in colour coded tubes, the set contains the DNA polymerase and a 10x concentrated proof-reading reaction buffer with 20 mM MgSO4.


De set bevat

ProofRead polymerase in storage buffer containing 50 % glycerol, PCR ProofRead buffer (10x) with 20 mM MgSO4.
Colour coded tubes.
Contents of this set may not be bought separately.



Geen medisch hulpmiddel / Geen ivD-product
Technische informatie
Toepassing High fidelity PCR with exact sequence replication 
Amplicon-einden blunt 
Polymerase Proof reading / high fidelity Pfu polymerase 
Reactiebuffer ProofRead 
Toepassing High fidelity PCR with exact sequence replication 
ROTI®Pol ProofRead
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00
Bestelnr. VE Verp. Verpakking Prijs Hoeveelheid
9344.1 40 µl plastic 1 x 40 µl

102,70 €

9344.2 200 µl plastic 5 x 40 µl

467,65 €

Op voorraad
Beschikbaar
In bestelling
Niet meer verkrijgbaar
Leveringsdatum onbekend
Geselecteerde hoeveelheid:   0
  1. Tussentotaal:  0.00

Downloads / MSDS


Algemene informatie

Enzyme: a neoclassical, Greek artificial word ενζυμου, énzymon, derived from εν-, en- (in-) and ζυμη, zýmé (yeast, sourdough, archaic)
Ferments: comes from the Latin fermentum (ferments, sourdough)

There are six classes in which all enzymes are classified according to the particular reaction they catalyse:

Oxidoreductases (catalyse redox reactions)

Transferases (transfer functional groups among substrates)

Hydrolases (cleave bonds via addition of water)

Lyases/Synthases (cleave or synthesise complex products out of basic substrates without cleavage of ATP)

Isomerases (transform chemical isomers)

Ligases/Synthetases (cleave or synthesise complex products out of basic substrates via cleavage of ATP)


Analysecertificaten

Hier kunt u uw analysecertificaat voor het geselecteerde product zoeken en downloaden. Geef een chargenummer op.
De volgende analysecertificaten werden gevonden:

Type analysis

Purity (enzyme)≥98 %
Enzyme activity5 U/µl
Endonuclease activity
Exonuclease activity
Suitability for PCR (400 bp)
Suitability for PCR (3 kb)
Selective specificity in PCR
Stability (in storage buffer)